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A Blood Test for Prion: Disease Associated Prion Aggregate is Detected in the Blood of Infected But Asymptomatic Animals Show Full Record 1798
Source: Clinical Vaccine Immunology. 2006 Nov 1 [Epub ahead of print] Resource Type(s): Journal (Articles) Searchable Keywords: Blood Chronic wasting disease Deer Detection Diagnosis Diagnostic testing Immunoassays PrPSc proteins Test developments Abstract: From abstract (online abstract only): We have developed a sensitive in vitro assay for detecting disease associated prion aggregates by combining an aggregation specific enzyme-linked immunosorbent assay (AS-ELISA) with a Fluorescent Amplification Catalyzed by T7 RNA polymerase Technique (FACTT). The new assay, named AS-FACTT, is much more sensitive than AS-ELISA and could detect prion aggregates in the brain of mice as early as 7 days after an intra-peritoneal inoculation of PrPSc. However, AS-...
A Case of Chronic Wasting Disease in an Elk Imported to Korea from Canada Show Full Record 390
Source: Journal of Veterinary Medical Science. 2002 Sep;64(9):855-8. Resource Type(s): Journal (Articles) Searchable Keywords: Canada Central nervous system Chronic wasting disease Deer Diagnosis Isolation & purification Korea Transportation (animal) Western blotting Abstract: From abstract (free full-text available): A seven-year-old male elk (Cervus elaphus nelsoni) was euthanized and necropsied after having a 3-week history of body weight loss, emaciation, excessive salivation, teeth grinding, fever, anorexia, and respiratory distress. The elk was imported into Korea from Canada on March 9, 1997. Gross pathologic findings were restricted to a diffuse fibrinous pneumonia. Microscopic lesions included mild neuronal vacuolation and spongiform change in the neuropil of...
A Multiplex RT-PCR for Detection of Type A influenza Virus and Differentiation of Avian H5, H7, and H9 Hemagglutinin Subtypes Show Full Record 1293
Source: Molecular and Cellular Probes. 2006 Mar 14; [Epub ahead of print] Resource Type(s): Journal (Articles) Searchable Keywords: Avian influenza Diagnosis Genetics Hemagglutinins Polymerase chain reaction Viral subtypes Abstract: From abstract (online abstract only): A multiplex reverse transcriptase-polymerase chain reaction (mRT-PCR) was developed and optimized for the detection of type A influenza virus; the assay simultaneously differentiates avian H5, H7 and H9 hemagglutinin subtypes. Four sets of specific oligonucleotide primers were used in this test for type A influenza virus, H5, H7 and H9 heamagglutinin subtypes. The mRT-PCR DNA products were visualized by gel electrophoresis and consisted of fragments of 860bp...
A Natural Case of Chronic Wasting Disease in a Free-ranging Moose (Alces alces shirasi) Show Full Record 2397
Source: Journal of Wildlife Disease. 2007 Apr; 43(2): 309-314 Resource Type(s): Journal (Articles) Searchable Keywords: Chronic wasting disease Colorado Diagnosis Immunochemistry Moose Western blotting Abstract: From abstract (online abstract only): Chronic wasting disease (CWD) was diagnosed in a free-ranging moose (Alces alces shirasi) killed by a hunter in Jackson County, Colorado, USA, in September 2005. The diagnosis was based upon immunohistochemistry (IHC) demonstrating the presence of accumulations of CWD-associated prion protein (PrP(CWD)) in tissue sections of medulla oblongata at the level of the obex (dorsal motor nucleus of the vagus) and in retropharyngeal lymph node (RPLN); additional tes...
A novel erythroid-specific marker of transmissible spongiform encephalopathies Show Full Record 296
Source: Nature Medicine. 2001; 7(3): 361-364 Resource Type(s): Journal (Articles) Searchable Keywords: Biological markers Chronic wasting disease Diagnosis Mice Prion diseases Abstract: From abstract(free full-text available): Transmissible spongiform encephalopathies (TSE) are a group of invariably fatal neurodegenerative diseases and include scrapie in sheep, bovine spongiform encephalopathy (BSE) in cattle, chronic wasting disease in deer and elk, and Kuru disease, Creutzfeldt-Jakob disease (CJD) and variant CJD in humans. The pathological effects of disease occur predominantly in the CNS (central nervous system), where common hallmarks include vacuolation, gliosis, accumula...
A Sensitive One-Step Real-Time PCR for Detection of Avian Influenza Viruses Using a MGB Probe and an Internal Positive Control Show Full Record 1375
Source: BMC Infectious Diseases. 2006 May 25;6(1):87 [Epub ahead of print] Resource Type(s): Journal (Articles) Searchable Keywords: Avian influenza Diagnosis Diagnostic techniques & procedures Polymerase chain reaction Abstract: From abstract (free full-text available) - BACKGROUND: Avian influenza viruses (AIVs) are endemic in wild birds and their introduction and conversion to highly pathogenic avian influenza virus in domestic poultry is a cause of serious economic losses as well as a risk for potential transmission to humans. The ability to rapidly recognise AIVs in biological specimens is critical for limiting further spread of the disease in poultry. The advent of molecular methods such as real time polymerase cha...
Anthrax (Bacillus anthracis) [Fact Sheet] Show Full Record 1706
Source: Georgia. Department of Agriculture Resource Type(s): Fact Sheets Searchable Keywords: Anthrax Diagnosis Disease transmission Prevention & control Zoonoses Abstract: Presents information about anthrax in a concise four-page fact sheet. Content includes differential diagnosis, suggestive necropsy findings, zoonotic risk, and potential as biothreat agent in humans/animals. Includes a list of over 15 electronic references.
Anthrax and Wildlife Show Full Record 1708
Source: Rev Sci Tech. 2002 Aug;21(2):359-83. Resource Type(s): Illustrations, Issue Overviews, Table or Spreadsheet Searchable Keywords: Africa Anthrax Bacillus anthracis Canada Diagnosis Epidemiology North America Pathology Abstract: From abstract (online abstract only): Although livestock anthrax is declining in many parts of the world, with an increasing number of countries probably truly free of the disease, anthrax remains enzootic in many national parks and even in some game ranching areas. These infected areas can present a persistent risk to surrounding livestock, which may otherwise be free of the disease, as well as a public health risk. The authors use as examples the national parks in southern Africa, the Wood Buf...
APHIS Veterinary Sevices - Chronic Wasting Disease Show Full Record 126
Source: U.S. Animal and Plant Health Inspection Service. Veterinary Services Resource Type(s): Bibliography and Web Indexes, Fact Sheets, Issue Overviews, Laws and Regulations, Maps Searchable Keywords: Captive cervids Chronic wasting disease Deer Diagnosis Game ranching Herd certification program History Legislation Prevention & control Regulations Transportation (animal) Abstract: Offers an overview of chronic wasting disease (CWD), as well as informational materials, such as a bibliography of scientific CWD articles, maps, fact sheets and updates on the management actions taken by USDA with the captive deer industry.
Avian Botulism in Birds - An Overview Show Full Record 484
Source: Canadian Cooperative Wildlife Health Centre Resource Type(s): Fact Sheets, Issue Overviews, Maps, Photographs Searchable Keywords: Avian botulism Diagnosis Disease transmission Epidemiology Etiology Prevention & control Wildlife management Abstract: Fact sheets on avian botulism, both Type C Botulism and Type E Botulism are presented Information includes, wildlife management aspects, the disease pathology and ecology, and affected bird species. |