Genetic Analysis in the Laboratory

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Since the early 1950's, laboratory methods to perform genetic analyses have rapidly advanced. Significant additions include the discovery of the three dimensional structure of DNA, technologies for sequencing and synthesizing DNA, and the development of laboratory reagents and procedures to identify expressed genes. Genetic analysis used to rely on the indirect correlation of genotype with phenotype (morphological characteristics). Now we can directly understand genotype at the molecular level.

Genetic Analysis
Showing 16 Results
CollapseAntigenic and Genetic Analysis of a Recently Isolated H1N1 Swine Influenza Virus
Description: From abstract (online abstract only): Hemagglutinins (HA) of H1N1 swine influenza viruses isolated in the United States have remained antigenically and genetically conserved for many years. In contrast to such conservation, the HA of A/Swine/Nebraska/1/92 (Sw/Neb) could readily be distinguished from those of contemporary porcine viruses. Twenty-eight amino acid mutations differentiated the HA of Sw/Neb and A/Swine/Indiana/1726/88, the most recent H1N1 swine influenza virus for which HA sequence data were available. Among these differences were mutations at potential asparagine-linked glycosylation sites and charge changes at many residues. The Sw/Neb virus also could be differentiated from other swine influenza viruses in hemagglutination-inhibition assays with monoclonal antibodies to recent H1 swine HA. Nonetheless, overall sequence analysis of the HA and the nucleoprotein genes of Sw/Neb indicated that this virus was more closely related genetically to classic H1N1 swine influenza viruses than to H1N1 avian or human viruses. Infection of swine with Sw/Neb under experimental conditions induced clinical signs and lesions typical of swine influenza. However, affected swine in the field had high, persistent fevers, but relatively mild signs of respiratory tract disease. This study indicated that an antigenically and genetically novel variant of swine influenza virus was detected in the United States.
Resource Type: Journal Articles
Resource Format: URL
Publisher: American Veterinary Medical Association
ExpandAntigenic and Genetic Analysis of a Recently Isolated H1N1 Swine Influenza Virus
ExpandAssessing family relationships in the broad-tailed hummingbird (Selasphorus platycercus)
ExpandDelaware Bay and Chesapeake Bay Populations of the Horseshoe Crab, Limulus polyphemus, are Genetically Distinct
ExpandForays into fungal genomics
ExpandGender identification of white-winged doves
ExpandHolistic Approach to Taxonomic Evaluation of Two Closely Related Endangered Freshwater Mussel Species, The Oyster Mussel Epioblasma capsaeformis and Tan riffleshell Epioblasma florentia walkeri (Bivalvia: Unionidae)(PDF, 17 pp., 699 KB)
ExpandMonitoring Grizzly Bear Populations using DNA in the Northern Divide Ecosystem
ExpandPatterns of Genetic Differentiation and Conservation of the Slabside Pearlymussel, Lexingtonia dolabelloides, in the Tennessee River Drainage
ExpandPhylogenetic Analysis of H7 Haemagglutinin Subtype Influenza A Viruses
ExpandPlant DNA Cis Elements (PLACE)
ExpandQuantifying and identifying unionid larvae in drift and on fishes of the Sipsey River, Alabama (PDF, 191.42 KB)
ExpandThe Chlamydomonas Genetics Center
ExpandWhite-Nose Syndrome Fungus (Geomyces destructans) in Bats, Europe (PDF, 6 pp., 234 KB)
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