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Laboratory techniques and available facilities/equipment:

 

One module in Building 10 9-D-17 fully equipped for molecular biology and basic biochemistry techniques. Full access to cell culture, imaging systems and radioactive isotopes laboratory.

Bench research techniques commonly employed: PCR, nucleic acid extraction and purification, cloning, cell culture (primary and stable cell lines), tranfection (transient and stable), Western Blot, Electrophoretic mobility shift assay, deiodinase activity assay.

 

Clinical research techniques commonly employed: hyperinsulinemic-euglycemic clamp, frequently sampled IV glucose tolerance test, TRH stimulation test, indirect calorimetry (hood and whole room), graded exercise tolerance test with evaluation of gas exchange, subcutaneous adipose tissue microdialysis, skeletal muscle and subcutaneous adipose tissue biopsy.

Ongloing Clinical Protocols

1) The role of peripheral conversion of thyroid hormones in the maintenance of glucose and energy metabolism (05-DK-0119)

 2) The effects of modulation of environmental temperature on the regulation of energy metabolism, substrate utilization and hormonal axes (07-DK-0202)

3) The role of macronutrients, gut-hormones and bile acids in the regulation of energy homeostasis and thermic effect of food (08-DK-0165)

4) The role of plant sterols in the modulation of markers of the metabolic syndrome (07-DK-0219)

 

Francesco Saverio Celi, M.D.


CEB
CLINICAL & EXPERIMENTAL THYROIDOLOGY SECTION
NIDDK, National Institutes of Health
Building 10-CRC , Room 6-3940
10 Center Dr.
Bethesda, MD 20892
Tel: 301-435-9267
Fax: 301-480-4517
Email: fc93a@nih.gov

Francesco Saverio Celi, M.D.

Research Statement:

The role of peripheral conversion of thyroid hormone in the modulation of thyroid hormone action: Dr. Celi’s research interest is aimed to the study of the role of the peripheral metabolism of tthyroid hormones, regulated by the deiodinases, in the modulation of the tissue-specificity of thyroid hormone action. Dr. Celi uses both in vitro and cell culture systems as well as human studies. The latter are mainly focused on the study of the interaction between peripheral metabolism of thyroid hormone and glucose, energy, and lipid homeostasis.


Selected Publications:

“Brown adipose tissue – when it pays to be inefficient” F.S. Celi. New England Journal of Medicine, April 2009;360:1553-56.

“The use of lithium as an adjuvant in the radioactive iodine treatment of thyroid cancer” M.S. Zemskova, F.S. Celi. In Victor R. Preedy, Gerard N. Burrow and Ronald Watson, editors: Comprhensive Handbook of Iodine, Oxford:Academic Press,2009, pp. 1001-1006.

“The role of type-1 and type-2 5’ deiodinase in the pathophysiology of the T3 toxicosis of McCune-Albright syndrome” F.S. Celi, G. Coppotelli, A. Chidakel, M. Kelly, B.A. Brillante, T. Shawker, N.Cherman, P.P. Feulillan, M.T. Collins. Journal of Clinical Endocrinology and Metabolism 2008; 93:2383-9.

“Multigenic control of thyroid hormone functions in the nervous system” J. Nunez, F.S. Celi, L. Ng, D. Forrest. Molecular and Cellular Endocrinology 2008 287:1-12.

“Thyroid storm-associated cholestatic jaundice. Report of two cases” K. Hull, R. Horenstein, R. Naglieri, K. Munir, M. Ghany, F.S. Celi. Endocrine Practice 2007;13: 476-480.

“Thyroid Disease in Patients with McCune-Albright Disease” V. Congedo, F.S. Celi Ped. Endocrinol. Rev. 2007 4 Suppl 4:429-33.

“Functional characterization of the 258 A/G (D2-ORFa-Gly3Asp) human type-2 deiodinase polymorphism. A naturally occurring variant increases the enzymatic activity by removing a putative repressor site in the 5’UTR of the gene” G. Coppotelli, A. Summers, A. Chidakel, J.M. Ross, F.S. Celi Thyroid 2006; 16:625-32.

“The Thr92Ala deiodinase type 2 polymorphism is not associated with indexes of insulin resistance in the old order of Amish” D. Mentuccia, M.J. Thomas, G. Coppotelli, L. Reinhart, B.D. Mitchell, A.R. Shuldiner F.S. Celi Thyroid. 2005;15:1223-7.

“Mutations in Gng3lg and AGPAT2 in Beradinelli-Seip Congenital Lipodystrophy and Brunzell Syndrome: Phenotype variability suggests important modifier effects” M. Fu, R. Kazlauskaite, M. de F.P. Baracho, M.G. Brandao-Neto, J. Villares, F.S. Celi, B.L. Wajchenberg, A.R. Shuldiner Journal of Clinical Endocrinology and Metabolism, 2004;89:2916-22.

“Association between a novel variant of the human type 2 deiodinase (DIO2) gene, Thr92Ala and insulin resistance: evidence of interaction with the Trp64Arg variant of the beta-3-adrenergic receptor (ADRB3)” D. Mentuccia, L. Proietti-Pannunzi, K. Tanner, V. Bacci, T.I. Pollin, E.T. Poehlman, A.R. Shuldiner, F.S.Celi DIABETES, 2002 Mar;51(3):880-3.

“Age-related prevalence of platelet-associated immunoglobulins g in nonthrombocytopenic patients with autoimmune thyroid disease and autoimmune polyglandular syndrome”. L. Gargano, D. Mentuccia, L. Conti, N. Viceconti, R. Sibilla, D. Danese, F.S.Celi, G. Gandolfo, M. Centanni. Horm Res. 2001;56(5-6):172-6.

“Increased expression of thyroid hormone receptor isoforms in end-stage human congestive heart failure” G. d’Amati, C.R.T.di Gioia, D. Mentuccia, D. Pistilli, L. Proietti-Pannunzi, F. Miraldi, P. Gallo, F.S.Celi Journal of Clinical Endocrinology and Metabolism 2001;86:2080-4.

“Preparing PolyA-containing RNA internal standards for multiplex competitive RT-PCR” F.S.Celi, D. Mentuccia, L. Proietti-Pannunzi, C.R.T. di Gioa, M. Andreoli Biotechniques 2000:29:454,457-8.

“Molecular scanning for mutations in the insulin receptor substrate-1 (IRS-1) gene in Mexican Americans with type 2 diabetes” F.S.Celi, C. Negri, K. Tanner, N. Raben, F. De Pablo, A. Rovira, L.F. Pallando, P. Marin-Vaguero, B.D. Mitchell, M.P. Stern, A.R. Shuldiner Diabetes and Metabolism Reviews 2000;16:370-7.

“Structural organization and chromosomal localization of the human type II deiodinase gene” F.S.Celi, G. Canettieri, D. Mentuccia, L. Proietti-Pannunzi, A. Fumarola, R. Sibilla, V. Predazzi, M. Ferraro, M. Andreoli, M. Centanni European Journal of Endocrinology 2000;143:267-71.

“ Isolation of the human type 2 deiodinase gene Promoter and characterization of a functional cyclic adenosine monophosphate response element” G. Canettieri, F.S.Celi, G. Baccheschi, L. Salvatori, M. Andreoli, M. Centanni Endocrinology 2000;141:1804-13.

“A rapid, simple and inexpensive step facilitates RNA extraction from whole blood cells” O. Gandini, F.S.Celi, M. Magnanti, P. Gazzaniga, I. Silvestri, B. Conti, L. Giuliani, D. Mentuccia, A.M. Aglianò Laboratory Investigation 1999;79:1731-2.

“Genomic characterization of the coding region of the human type II 5’-deiodinase gene” F.S.Celi, G. Canettieri, D.P. Yarnall, D.K. Burns, M.Andreoli, A.R. Shuldiner, M. Centanni Molecular and Cellular Endocrinology 1998;141:49-52.

"Lack of IRS-1 Codon 513 and 972 polymorphism in Pima indians" F.S.Celi, K.Silver, J.Walston, W.C.Knowler, C.Bogardus, A.R.Shuldiner Journal of Clinical Endocrinology and Metabolism 1995;80:2827-9.

"Time of onset of non-insulin-dependent diabetes mellitus and genetic variation of the B3-adrenergic-receptor gene" J.Walston, K.Silver, C.Bogardus, W.C.Knowler, F.S.Celi, S.Austin, B.Manning, D.Strosberg, M.P.Stern, N.Raben, J.Sorkin, J.Roth, A.R.Shuldiner New England Journal of Medicine, August 1995;333:343-7.



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