Primer-BLAST was developed at NCBI to help users make primers that are specific
 to intended PCR target.  It uses Primer3 to design PCR primers and then uses 
BLAST and global alignment algorithm to screen primers against user-selected database
in order to avoid primer pairs (all combinations including forward-reverse primer pair,
 forward-forward as well as reverse-reverse pairs) that can cause non-specific amplifications. 
To cite Primer-BLAST or look for more details, please consult our publication:

Ye J, Coulouris G, Zaretskaya I, Cutcutache I, Rozen S, Madden T (2012).
Primer-BLAST: A tool to design target-specific primers for polymerase chain reaction.
BMC Bioinformatics. 13:134.
 

Use of Primer3 is subject to the following terms and conditions:


Copyright Notice and Disclaimer
Copyright (c) 1996,1997,1998,1999,2000,2001,2004,2006,2007
Whitehead Institute for Biomedical Research, Steve Rozen, and Helen Skaletsky
All rights reserved. 

Redistribution and use in source and binary forms, with or without
modification, are permitted provided that the following conditions are
met:

   * Redistributions of source code must retain the above copyright
notice, this list of conditions and the following disclaimer.
   * Redistributions in binary form must reproduce the above
copyright notice, this list of conditions and the following disclaimer
in the documentation and/or other materials provided with the
distribution.
   * Neither the names of the copyright holders nor contributors may
be used to endorse or promote products derived from this software
without specific prior written permission.

THIS SOFTWARE IS PROVIDED BY THE COPYRIGHT HOLDERS AND CONTRIBUTORS
"AS IS" AND ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT
LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR
A PARTICULAR PURPOSE ARE DISCLAIMED. IN NO EVENT SHALL THE COPYRIGHT
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(INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE
OF THIS SOFTWARE, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE.


Steve Rozen and Helen J. Skaletsky (2000)  Primer3 on the WWW for general users and for biologist programmers.
In: Krawetz S, Misener S (eds) Bioinformatics Methods and Protocols: Methods in Molecular Biology.
Humana Press, Totowa, NJ, pp 365-386