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Technical Laboratory Workshop: Antimicrobial Efficacy Test Methods and Activities November 13-15, 2012
Dates:
- November 13 -14, 2012 8:30 a.m. to 4:00 p.m
- November 15 8:30 a.m. to 12:00 p.m.
Where:
Environmental Protection Agency’s Environmental Science Center
701 Mapes Road
Ft. Meade, MD 20755-5350
Registration:
Registration Form (PDF) (2 pp, 153.02 K, About PDF)
There is no registration fee.
Registered participants will be provided information about the safety requirements for entering the labs, directions and security requirements, local hotels, and other logistics information.
Submit requests to participate in the meeting to
Michele Cottrill,
Office of Pesticide Programs,
Environmental Protection Agency,
701 Mapes Road,
Ft. Meade, MD 20755;
telephone number (410) 305-2955;
fax number (410) 305-3904;
cottrill.michele@epa.gov.
Summary
The U.S. EPA invites interested stakeholders to participate in a laboratory-based technical workshop that will focus on several key test methods for evaluating the efficacy of disinfectants. The agency is encouraging participation from a cross-section of affected parties including industry, federal and state partners, and testing laboratories.
The sessions will cover the AOAC Confirmative in vitro Test for Determining Tuberculocidal Activity (AOAC Official Method 965.12 II) and the new Organization for Economic Cooperation and Development (OECD) Quantitative Method for Evaluating Bactericidal Activity of Microbicides Used on Hard Non-porous Surfaces.
Demonstrations and training will be conducted in a laboratory setting on the revised Use-dilution method (AOAC Official Methods 955.15 and 964.02) and the new proposed ASTM Quantitative Petri Dish (QPM) method for evaluating the efficacy of antimicrobial wipes. In addition, editorial and procedural revisions to other efficacy test methods will be discussed. The results from the recent collaborative study on the OECD method will also be covered. Stakeholders are expected to actively participate by providing relevant data and sharing their experiences working with these test methods.
This is a technical meeting for individuals with experience in conducting antimicrobial test methods or who plan to conduct these methods in the future.
Participants must be knowledgeable of Biosafety Level II and III safety practices and procedures for working in a laboratory in order to participate in the laboratory demonstrations and training.
Agenda
2012 Technical Workshop on Antimicrobial Efficacy Test Methods and Activities – Day 1
November 13 (AM)
- Introductions and Agenda
- Lab safety practices and PPE
- Recent revisions to the method – AOAC method 965.12
- CTB Laboratory Session 1
- Carriers – CTB and GSPT
- Screening/use
- Storage
- Media – preparation sheets
- Test Microbe
- Mycobacterium bovis BCG; source(s)
- Diagnostic characteristics
- Establishment of stock cultures and test cultures – key topic
- Potential culture viability issues
- Conducting the CTB
- Setup
- Harvesting and standardizing the culture – key topic
- Dropping/spraying carriers
- Control carrier counts – key topic
- Incubation
- Recording results
- Lunch
November 13 (PM)
- CTB Laboratory Session 2
- CTB Media Performance Assessment – key topic
- Growth in liquid media
- Decision making process
- Use of agitated cultures – alternative to static cultures
- OECD Test Guidelines for Mycobacterium
- Method Overview
- Mycobacterium terrae/test culture preparation
- Carrier screening/quality
- Growth media/culture harvest/stocks
- Hard water and soil preparation
- Carrier inoculation
- Exposure/neutralization/filtration
- Results
- Neutralization Assay
- Day 1 Wrap-up
- Tabulate issues and concerns
- Revisions and action items
- Collaborative studies
- Next steps
2012 Technical Workshop on Antimicrobial Efficacy Test Methods and Activities – Day 2
November 14 (AM)
- Introductions and Agenda
- 2012 AOAC Committee M Activities
- Concurrent UDM discussion session (Group 1)
- Editorial changes
- Procedural changes
- Performance standard
- Stakeholder presentation/input
- Concurrent UDM lab session (Group 2)
- Demonstrations and revision walk-through
- Concurrent UDM discussion session (Group 2)
- Editorial changes
- Procedural changes
- Performance standard
- Stakeholder presentation/input
- Concurrent UDM lab session (Group 1)
- Demonstrations and revision walk-through
- Lunch
November 14 (PM)
- Discussion session
- CTB revisions
- Germicidal Spray Test Revisions
- Stakeholder presentation/input
- OECD Test Guidelines
- Results of the collaborative study
- OECD’s timeline
- EPA’s implementation strategy
- Stakeholder presentation/input
- New Methods (biofilms, C. difficile etc.)
- Day 2 Wrap-up
2012 Technical Workshop on Antimicrobial Efficacy Test Methods and Activities – Day 3
November 15 (AM)
- Quantitative Testing of Towelettes – the QPM
- Background
- ASTM process to date
- Method overview – ASTM Standard
- Laboratory session
- Method demonstration and group (2) training
- Collaborative study
- Goals
- Test design
- Treatment selection
- Timeline
- Day 3 wrap-up
- Tabulate action items
- Next steps
- Adjourn
Workshop Announcement Flyer (5 pp, 384K, About PDF)