Amendments and Corrections to RFA-HG-05-007
of a Comprehensive Mouse Knockout Resource”
Letters of Intent Due: October 20, 2005
Applications Due: November 22, 2005
The purpose of this RFA is to solicit applications for research projects to
initiate a trans-NIH program for the production of a comprehensive resource
of mouse mutants in which every gene in the mouse genome has been knocked out
by a null mutation marked with a reporter system of high utility. The ideal
resource would be one in which all of the mutations are carried on a uniform
background in strain C57BL/6, which is the strain most widely utilized by mouse
researchers. But the NIH recognizes that, at present, there are certain technical
problems, primarily related to low efficiency, with the use of C57BL/6 as the
background strain for this type of high-throughput project. Therefore, the
primary focus of this solicitation will be on construction of the mutations,
and a secondary focus will be on the choice of mouse stain. However, applications
that propose approaches to the cost-efficient generation of the mutations directly
in strain C57BL/6 will be given preference for funding.
This RFA is being issued to further the value of the mouse as a powerful and
important tool in the study of human disease. For many years, mouse mutants
with phenotypes that mimic human traits have served as critical research tools
in understanding the genetics underlying mammalian biology. The importance
of the mouse as a model organism was indicated by the inclusion of a goal for
the construction of genetic and physical maps of the mouse genome in the initial
plan for the Human Genome Project (HGP). In fact, the HGP was actually able
to accomplish significantly more than generating such maps of the mouse genome,
and a high quality, finished sequence of the mouse genome (strain C57BL/6)
will be completed by the end of 2005. Another major genomic resource for mouse
research has been developed by the Mammalian Gene Collection (MGC) project
which as of July 2005 included 15,325 full-ORF (open reading frame) cDNA clones,
representing 11,501 individual mouse genes. The goal of the MGC mouse
cDNA program is to produce, by 2007, at least one full-ORF cDNA clone for each
of the ~18,000 currently well-defined mouse genes.
This funding opportunity will use the cooperative agreement award mechanism(s).
Up to $50 million in total costs over 5 years will be awarded through this RFA.
It is anticipated that 1 to 4 awards will be made in July 2006 for up to five
years. The full text of the RFA is available at: http://grants.nih.gov/grants/guide/rfa-files/RFA-HG-05-007.html.