En EspanolTalking Glossary of Genetic Terms
Stacie Loftus, Ph.D. defines Northern Blot
Northern Blot
Northern blot is a laboratory technique used to detect a specific RNA sequence in a blood or tissue sample. The sample RNA molecules are separated by size using gel electrophoresis. The RNA fragments are transferred out of the gel to the surface of a membrane. The membrane is exposed to a DNA probe labeled with a radioactive or chemical tag. If the probe binds to the membrane, then the complementary RNA sequence is present in the sample.
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