Forensic DNA: Miniaturization and Automation
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Description
New technologies are being developed to miniaturize DNA testing instruments and to improve automation of the processes involved. Both miniaturization and automation have the potential to improve the speed and to reduce the cost of DNA analysis. In addition, miniaturizing the instrumentation required to perform DNA testing could enable examination of biological samples at the crime scene if desired in the future here in the United States.
Research Projects
The table below presents awards funded both prior to and under the DNA Initiative, which began in 2004.
| Title | Awardee | Amount | Award Number |
|---|---|---|---|
| Use of Novel Chemistry & Microwave Instrumentation to ImproveBody Fluid Assay Sensitivity & Speed while Reducing Costs | City of New York, Office of Chief Medical Examiner | 2012-DN-BX-K044 | $403,671 |
| DNA Analysis of LCN Samples: Towards Fully Integrated STR Profiling | Network Biosystems, Inc | 2009-DN-BX-K256 | $496,991 |
| A Low Cost Microfluidic Instrument for Typing SNPs | Akonni Biosystems Inc | 2009-DN-BX-K189 | $495,031 |
| Microchip Analyzer for Forensic Short Tandem Repeat Typing of Single Cells | Regents of the University of California - Berkeley | 2009-DN-BX-K180 | $241,117 |
| Closed System DNA Purification for Degraded and Compromised Evidence in Microfluidic Devices | University of Virginia | 2008-DN-BX-K144 | $480,000 |
| Sample Collection Systems for DNA Analysis of Forensic Evidence Samples | Network Biosystems, Inc. | 2008-DN-BX-K010 | $498,563 |
| Human Specific DNA Quantitation for Fully Integrated Microfluidic STR Analysis | Network Biosystems Inc. | 2008-DN-BX-K009 | $494,319 |
| Microfluidic DNA Extraction and Purification from Forensic Samples: Towards Rapid, Full Integrated STR Analysis | Network Biosystems, Inc. | 2007-DN-BX-K184 | $497,346 |
| A Low-Cost Microfluidic Microarray Instrument for Typing Y-Chromosome Single Nucleotide Polymorphisms (SNPs) | Akonni Biosystems, Inc. | 2007-DN-BX-K145 | $448,466 |
| Microfabricated Capillary Array Electrophoresis Genetic Analysis for Forensic Short Tandem Repeat DNA Profiling | Regents of the University of California | 2007-DN-BX-K142 | $592,183 |
| Separation of Sperm and Epithelial Cells in a Microfluidic Device: An Automated Method for High-Efficiency, High-Purity Separations | University of Virginia | 2006-DN-BX-K021 | $324,104 |
| SNP-Based Microarray Technology for Use in Forensic Applications | Affymetrix, Inc. | 2006-DN-BX-K021 | $638,098 |
| Analysis of DNA Forensic Markers Using High Throughput Mass Spectrometry | Isis Pharmaceuticals, Inc. | 2006-DN-BX-K011 | $451,382 |
| SNP-Based Microarray Technology for Use in Forensic Applications | Affymetrix, Inc. | 2005-DA-BX-K101 | $638,098 |
| Electro-Elution Assisted Purification of DNA for Forensic Analysis | University of Virginia | 2005-DA-BX-K100 | $20,000 |
| Nanotechnology DNA Sequencing: Improving DNA Processing Technologies | Brown University | 2004-LT-BX-K001 | $445,265 |
| Nanotechnology DNA Sequencing: Improving DNA Processing Technologies | Brown University | 2004-LT-BX-K001 | $542,654 |
| Microfabricated Capillary Array Electrophoresis Genetic Analyzers for Forensic Short Tandem Repeat DNA Profiling | University of California | 2004-DN-BX-K216 | $821,623 |
| Laser Microdissection Separation of Pure Spermatozoa Populations From Mixed Cell Samples for Forensic DNA Analysis | Rosalind Franklin University of Medicine and Science | 2004-DN-BX-K215 | $449,829 |
| A Hand-Held DNA-Based Forensic Tool | Cornell University | 2004-DN-BX-K001 | $644,251 |
| Application of a Single Robotics Platform to the Combined Extraction, Purification, Quantification, and Amplification of Forensic DNA Samples | North Louisiana Criminalistics Laboratory | 2002-IJ-CX-K019 | $42,401 |
| Program Acceleration and Enhancements for Microdevice DNA Forensics System | Whitehead Institute for Biomedical Research | 1998-LB-VX-K022 | $149,964 |
| Microdevice for Automated, Ultra-High-Speed, and Portable DNA Forensics | Whitehead Institute for Biomedical Research | 1998-LB-VX-K022 | $250,000 |
| Program Acceleration/Enhancements for Microdevice DNA Forensics System | Whitehead Institute for Biomedical Research | 1998-LB-VX-K022 | $499,342 |
| Microdevice for Automated, Ultra-High-Speed, and Portable DNA Forensics | Whitehead Institute for Biomedical Research | 1998-LB-VX-K022 | $899,110 |
| Microdevice for Automated, Ultra-High-Speed, and Portable DNA Forensics | Whitehead Institute for Biomedical Research | 1998-LB-VX-K022 | $1,102,790 |
| Microdevice for Automated, Ultra-High-Speed, and Portable DNA Forensics | Whitehead Institute for Biomedical Research | 1998-LB-VX-K022 | $2,486,876 |
| Chip-Based Genetic Detector for Rapid Identification of Individuals | Nanogen, Inc. | 1997-LB-VX-0004 | $224,110 |
| Chip Based Genetic Detector for Rapid Identification of Individuals | Nanogen, Inc. | 1997-LB-VX-0004 | $499,882 |
| Chip-Based Genetic Detector for Rapid Identification of Individuals | Nanogen, Inc. | 1997-LB-VX-0004 | $514,848 |
| Chip Based Genetic Detector for Rapid Identification of Individuals | Nanogen, Inc. | 1997-LB-VX-0004 | $709,919 |
| Chip-Based Genetic Detector for Rapid Identification of Individuals | Nanogen, Inc. | 1997-LB-VX-0004 | $799,692 |
| Chip-Based Genetic Detector of Rapid Identification of Individuals | Nanogen, Inc. | 1997-LB-VX-0004 | $1,700,000 |
| Improved Analysis of DNA Short Tandem Repeats for Human Identification | Genetrace Systems, Inc. | 1997-LB-VX-0003 | $301,999 |
| Improved Analysis of DNA Short Tandem Repeats for Human Identification | Genetrace Systems, Inc. | 1997-LB-VX-0003 | $307,664 |
Research Articles and Reports
Select a title to view an abstract from PubMed database (National Library of Medicine's) unless a different file type is indicated.
| Title | Authors | Publication | Year | Awardee | Award Number |
|---|---|---|---|---|---|
| A Low Cost Microfluidic Microarray System for Typing Y Chromosome SNPs (pdf, 45 pages) | Jennifer E. Reynolds, Ph.D., Phil Belgrader Ph.D., Rebecca Holmberg Ph.D., Cynthia, Zimmerman, Clare Stewart, Rebecca Mokhiber, and Christopher Cooney, Ph.D. | Final Grant Report | 2009 | Akonni Biosystems, Inc. | 2007-DN-BX-K145 |
| A Hand-Held DNA-Based Forensic Tool (pdf, 55 pages) | Carl A. Batt, Scott J. Stelick, Matthew J. Kennedy, Clarissa S. Lui, Adam J. Lowe | Final Grant Report | 2009 | Cornell University | 2004-DN-BX-K001 |
| Separation of Sperm and Epithelial Cells in a Microfluidic Device: An Automated Method for High Efficiency, High Purity Separations (pdf, 71 pages) | James P. Landers, Ph.D. | Final Grant Report | 2009 | University of Virginia | 2006-DN-BX-K021 |
| Integrated sample cleanup-capillary electrophoresis microchip for high-performance short tandem repeat genetic analysis. | Yeung SH, Liu P, Del Bueno N, Greenspoon SA, Mathies RA. | Anal Chem. 2009 Jan 1;81(1):210-7. | 2009 | University of California, Berkeley | 2007-DN-BX-K142 |
| Microfluidic DNA Extraction and Purification from Forensic Samples (pdf, 88 pages) | Eugene Tan, Heidi Giese, and Daniel M. Hartmann | Final Grant Report | 2009 | Network Biosystems, Inc. | 2007-DN-BX-K184 |
| A forensic laboratory tests the Berkeley microfabricated capillary array electrophoresis device | Greenspoon SA, Yeung SH, Johnson KR, Chu WK, Rhee HN, McGuckian AB, Crouse CA, Chiesl TN, Barron AE, Scherer JR, Ban JD, Mathies RA. | J Forensic Sci. 2008 Jul;53(4):828-37 | 2008 | University of California, Berkeley | 2004-DN-BX-K216 |
| Real-time forensic DNA analysis at a crime scene using a portable microchip analyzer | Peng Liu, Stephanie H.I. Yeung, Karin A. Crenshaw, Cecelia A. Crouse, James R. Scherer, Richard A. Mathies | Forensic Science International: Genetics September 2008 (Vol. 2, Issue 4, Pages 301-309) |
2008 | University of California, Berkeley | 2004-DN-BX-K216 |
| Integrated portable polymerase chain reaction-capillary electrophoresis microsystem for rapid forensic short tandem repeat typing. | Liu P, Seo TS, Beyor N, Shin KJ, Scherer JR, Mathies RA. | Anal Chem. 2007 Mar 1; 79(5):1881-9. Epub 2007 Feb 2. | 2007 | University of California, Berkeley | 2004-DN-BX-K216 |
| Development of Microdevice Solid-Phase Purification Utilizing Dual Pressure/ Electro-Elution for Concentration and Enhanced Recovery of DNA (pdf, 24 pages) | Joan Bienvenue | Final Grant Report | 2007 | University of Virginia | 2005-DA-BX-K100 |
| Forensic application of the Affymetrix human mitochondrial resequencing array | Vallone, P.M., Jakupciak, J.P., Coble, M.D. | Forensic Science International: Genetics June 2007 (Vol. 1, Issue 2, Pages 196-198) |
2007 | NIST | 2003-IJ-R-029 |
| A Chip-Based Genetic Detector for Rapid Identification of Individuals (pdf, 102 pages) | Ron Sosnowski | Final Grant Report | 2006 | Nanogen, Inc. | 1997-LB-VX-0004 |
| Rapid and high-throughput forensic short tandem repeat typing using a 96-lane microfabricated capillary array electrophoresis microdevice. | Yeung SH, Greenspoon SA, McGuckian A, Crouse CA, Emrich CA, Ban J, Mathies RA. | J Forensic Sci. 2006 Jul; 51(4):740-7. | 2006 | University of California | 2004-DN-BX-K216 |
| Microdevice DNA forensics by the simple tandem repeat method. | Goedecke N, McKenna B, El-Difrawy S, Gismondi E, Swenson A, Carey L, Matsudaira P, Ehrlich DJ. | J Chromatogr A. 2006 Apr 14; 1111(2):206-13. | 2006 | Whitehead Institute for Biomedical Research | 1998-LB-VX-K022 |
| Surface modification of poly(methyl methacrylate) for improved adsorption of wall coating polymers for microchip electrophoresis. | Shah JJ, Geist J, Locascio LE, Gaitan M, Rao MV, Vreeland WN. | Electrophoresis. 2006 Oct; 27(19):3788-96. | 2006 | NIST | 2003-IJ-R-029 |
| Cellular immobilization within microfluidic microenvironments: dielectrophoresis with polyelectrolyte multilayers. | Forry SP, Reyes DR, Gaitan M, Locascio LE. | J Am Chem Soc. 2006 Oct 25; 128(42):13678-9. | 2006 | NIST | 2003-IJ-R-029 |
| Forensic DNA typing by capillary electrophoresis using the ABI Prism 310 and 3100 genetic analyzers for STR analysis. | Butler JM, Buel E, Crivellente F, McCord BR. | Electrophoresis. 2004 Jun; 25(10-11):1397-412. | 2004 | National Institute of Standards and Technology | 1999-IJ-R-A094 |
| Genotyping SNPs Using a UV-Photocleavable Oligonucleotide in MALDI-TOF MS. | Vallone PM, Fahr K, Kostrzewa M. | Methods Mol Biol. 2004; 297:169-78. | 2004 | National Institute of Standards and Technology | 1999-IJ-R-A094 |
| Multiple sample amplification and genotyping integrated on a single electronic microarray. | Huang Y, Shirajian J, Schroder A, Yao Z, Summers T, Hodko D, Sosnowski R. | Electrophoresis. 2004 Oct: 25(18-19): 3106-16 | 2004 | Nanogen, Inc. | 1997-LB-VX-0004 |
| A high-performance multilane microdevice system designed for the DNA forensics laboratory. | Goedecke N, McKenna B, El-Difrawy S, Carey L, Matsudaira P, Ehrlich D. | Electrophoresis. 2004 Jun; 25(10-11):1678-86. | 2004 | Whitehead Institute for Biomedical Research | 1998-LB-VX-K022 |
| High-speed analysis of multiplexed short tandem repeats with an electrophoretic microdevice. | Mitnik L, Carey L, Burger R, Desmarais S, Koutny L, Wernet O, Matsudaira P, Ehrlich D. | Electrophoresis. 2002 Mar; 23(5):719-26. | 2002 | Whitehead Institute for Biomedical Research | 1998-LB-VX-K022 |
| Rapid, high fidelity analysis of simple sequence repeats on an electronically active DNA microchip. | Radtkey R, Feng L, Muralhidar M, Duhon M, Canter D, DiPierro D, Fallon S, Tu E, McElfresh K, Nerenberg M, Sosnowski R. | Nucleic Acids Res. 2000 Apr 1; 28(7):E17. | 2000 | Nanogen, Inc. | 1997-LB-VX-0004 |
| Reliable genotyping of short tandem repeat loci without an allelic ladder using time-of-flight mass spectrometry. | Butler JM, Li J, Shaler TA, Monforte JA, Becker CH. | Int J Legal Med. 1999; 112(1):45-9 | 1999 | Genetrace Systems, Inc. | 1997-LB-VX-0003 |
| Two-color multiplexed analysis of eight short tandem repeat loci with an electrophoretic microdevice. | Schmalzing D, Koutny L, Chisholm D, Adourian A, Matsudaira P, Ehrlich D. | Anal Biochem. 1999 May 15; 270(1):148-52. | 1999 | Whitehead Institute for Biomedical Research | 1998-LB-VX-K022 |
| Single nucleotide polymorphism determination using primer extension and time-of-flight mass spectrometry. | Li J, Butler JM, Tan Y, Lin H, Royer S, Ohler L, Shaler TA, Hunter JM, Pollart DJ, Monforte JA, Becker CH. | Electrophoresis. 1999 Jun; 20(6):1258-65. | 1999 | Genetrace Systems, Inc. | 1997-LB-VX-0003 |
Date Modified: October 11, 2012
