such-1 and gfi-3 transcriptional fusions are expressed predominantly in the adult germline and embryo. such-1∷mCherry∷H2B∷such-1 and gfi-3∷mCherry∷H2B∷gfi-3 stable transgenes were imaged by fluorescence microscopy (A–H) and DIC (B, C, F, and G). such-1 (A) and gfi-3 (E) adult hermaphrodites exhibit expression in the germline extending from the distal tip (inset) to the −1 oocyte (arrowhead, A and E). Sperm expression is observed in the spermathecae (arrow, A and E). Somatic gut nuclei (asterisk, E) also express gfi-3. such-1 is expressed in meiotic (arrow, B) and mitotic embryos (C). gfi-3 is expressed at all embryonic stages, including meiosis (arrow, F) and mitosis (G). Male germlines also express the such-1 (D) and gfi-3 (H) transgenes; mature sperm are indicated with an arrow. Bars, 10 μm.