The National Institute of Diabetes & Digestive & Kidney Diseases

The Laboratory’s main interest is production and purification of biological products from both prokaryotes and eukaryotes of native and recombinant strains. Our work involves all aspects of this process including research, development, and production. To accomplish this mission, the Laboratory includes a pilot production facility that is well-equipped with recovery and purification equipments and bioreactors of various sizes and configurations for propagation of biological systems such as mammalian cells, insect cells, bacteria, yeast, and fungi. The biological products we make support clinical and structural studies.

The Laboratory conducts research and process development based on growth optimization, production scale-up and product recovery processes. We concentrate on protein production and purification with an emphasis on scaling up. One of the current research topics is aimed to achieve better understanding of the growth behavior and metabolism of both E. coli and several mammalian cell lines. The objective is to be able to overcome specific difficulties in the growth and the production process by modifying the properties of the producers. This work involved with gene transcription and expression analysis as well as cells transfection and mutation. Our recent achievement is identifying genes involve in mammalian cell adhesion, which allow us to modify the way the cells grow.

Druz A, Chia C, Majors B, Santuary R, Betenbaugh M, Shiloach J. A novel microRNA mmu-mir-466h affects apoptosis regulation in mammalian cells. Biotechnology and Bioengineering. 108(7);1651-1661, 2011. [Full Text/Abstract]

Chu C, Lugovtsev V, Leuis A, Betenbaugh M, Shiloach J. Production and antigenic properties of influenza virus from suspension MDCK–siat7e cells in a bench-scale bioreactor. Vaccine. 28(44);7193-7201, 2010. [Full Text/Abstract]

Negrete A, Ng WI, Shiloach J. Glucose uptake regulation in E. coli by the small RNA SgrS: comparative analysis of E. coli K-12 (JM109 and MG1655) and E. coli B (BL21). Microbial Cell Factories 9;75, 2010. [Full Text/Abstract]

Shiloach J, Reshamwala S, Noronha SB, Negrete A. Analyzing metabolic variations in different bacterial strains, historical perspectives and current trends- example E. coli. Current Opinion in Biotechnology. 21(1);1-6, 2010. [Full Text/Abstract]

Chu C, Lugovtsev V, Golding H, Betenbaugh M, Shiloach J. Conversion of MDCK cell line to suspension culture by transfecting with human siat7e gene and its application for influenza virus production. Proceedings of the National Academy of Sciences USA 106;14802-14807, 2009. [Full Text/Abstract]

Shiloach J, Rinas U. Glucose and acetate metabolism in E. coli-system level analysis and biotechnological applications in protein production processes. In: Lee SY, ed. System Biology and Biotechnology of E. coli. Springer; 377-400, 2009.

Chill L, Trinh L, Azadi P, Ishihara M, Sonon R, Karnaukhova K, Ophir Y, Golding B, Shiloach J. Production, purification, and characterization of human α1 proteinase inhibitor from Aspergillus. Biotechnology and Bioengineering. 102(3);828-844, 2009. [Full Text/Abstract]

Jaluria P, Betenbaugh M, Konstantopoulos K, Frank B, Shiloach J. Application of microarrays to identify and characterize genes involved in attachment dependence in HeLa cells. Metabolic Engineering. 9(3);241-257, 2007. [Full Text/Abstract]

Jaluria P, Chu C, Betenbaugh M, Shiloach J. Cells by design: a mini-review of targeting cell engineering using DNA microarrays. Molecular Biotechnology. 39(2);105-111, 2008. [Full Text/Abstract]

Phue JN, Lee SJ, Trinh L, Shiloach J. Modified E. coli B (BL21), a superior producer of plasmid DNA compared with E. coli K (DH5α). Biotechnology and Bioengineering. 101(4);831-836, 2008. [Full Text/Abstract]

Phue Je-Nie, Kedem B, Jaluria P, Shiloach J. Evaluating microarrays using semiparametric approach: Application to the entral carbon metabolism of E. coli BL21and JM109. Genomics. 89(2);300-305, 2007. [Full Text/Abstract]

Jaluria P, Betenbaugh M, Konstantopoulos K, Shiloach J. Enhancement of cell proliferation in various mammalian cell lines by gene insertion of a cyclin-dependent kinase homolog. BMC Biotechnology. 7;71, 2007. [Full Text/Abstract]

Shiloach J, Fass R. Growing E.coli to high cell density — a historical perspective on method development. Biotechnology Advances. 23(5);345-357, 2005. [Full Text/Abstract]