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Annotation Examples

NOTE: The BankIt Submission Tool now uses MyNCBI to authenticate logins. Previous BankIt users who have exisiting NCBI PDA login accounts should use the same PDA Username and Password to sign in to MyNCBI now.

If you have any questions about using the BankIt sequence submission tool, please contact GenBank User services at: and use 'BankIt' and your BankIt ID# in the subject line.

Be aware of BankIt's new features and submitting options:

  1. Once a submitter registers to use BankIt, the submitter's contact information is saved and is automatically displayed each subsequent time the submitter logs in to submit
  2. BankIt can be used to submit both a single sequence and a set of sequences in one submission
  3. BankIt accepts source qualifier and sequence feature data both as text input and as uploaded files
  4. BankIt allows a submitter to stop in the middle of a submission and to continue with that submission at a later time
  5. BankIt allows submitters to use the tabs at the top of the submission input pages to navigate and to edit previously visited pages.

GenBank Submission Options

Use BankIt if:
  • you have a single sequence, a simple set of sequences (for example:16S rRNA, matK, ITS/rRNA, amoE, tefB, cytb, or COI sets), or a small batch of different sequences
  • you prefer to use a web-based submission tool
  • the feature annotation for your sequences is not complicated
  • you do not require advanced sequence analysis tools
Use Sequin if:
  • you prefer to work on your submission off-line
  • you have a sequence or sequences that are complex
  • you would like graphical viewing and editing options, including an alignment editor
  • you would like the option to have network access to related analytical tools

How does BankIt work with MyNCBI login system?

MyNCBI allows you to create an account that will act as your gateway to NCBI's databases and submission tools, such as the BankIt Submission Tool. MyNCBI associates your BankIt submissions with your login name and remembers your contact information to expedite future BankIt submissions. The BankIt tool also associates your most recent incomplete submission with your MyNCBI login name; if you're interrupted while submitting a BankIt submission, you can return to the tool to complete the submission later.

To register through MyNCBI, follow the 'Sign in to use BankIt' link at the top of the BankIt homepage and click the 'Register for an account' link on the left side of the resulting MyNCBI Sign In page.

To Sign in, enter your Username and Password on that same page after you have completed the registration process. NOTE: Previous BankIt users who have exisiting NCBI PDA login accounts should use the same PDA username and password to sign in to MyNCBI now.

In order to ensure that the user currently using the BankIt submission tool is the person submitting the BankIt submission, you will be prompted for your MyNCBI user name and password before you begin a BankIt submission.

GenBank Sequence Submission Policy

  1. the GenBank database is intended for new sequence data that is determined by and annotated by the submitter
  2. sequences built or derived from other GenBank primary data intended for the Third Party Annotation (TPA) database may be submitted through BankIt
  3. the following types of submissions are NOT acceptable:
    • sequences less than 200 nucleotides long, unless they represent complete exons, non-coding RNAs (ncRNAs), microsatellites or ancient DNA
    • non-contiguous sequences that have been artificially joined; for example, multiple exons without their intervening introns or without a 'gap' of internal NNNs representing any missing sequence
    • primer-only sequences
    • protein-only sequences
    • single sequences that are a mix of molecule types, such as mix of genomic and mRNA sequence data
    • Expressed Sequence Tags (ESTs; these should be submitted through the dbEST system)
    • Genome Survey Sequences (GSSs; these should be submitted through the dbGSS system)
    • Sequence Tagged Sites (STSs; these should be submitted through the dbSTS system)

What is needed to submit a sequence through BankIt?

(See Submission Requirements page for more detail)

  • registration through the MyNCBI Login System (register on Sign in page through link above)
  • sequence data can be either cut-and-pasted as text or uploaded as file (multiple sequences must be in a FASTA format)
  • date for public release (immediate or at a specified future date)
  • basic information (authors and a working title) for a corresponding reference paper
  • name(s) of the organism(s) from which the sequence data were isolated and any other related descriptive data
  • sequence features (for example: CDS, gene, rRNA, tRNA, with nucleotide intervals and product names)

Updates to Sequences that are already submitted

BankIt does not have an update option. Please see options for updating a submitted or existing GenBank record.