NIH Library Training Training Description

Training Description

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Sequence Similarity Search: BLAST-Like Alignment Tool (BLAT)

Description:

The BLAST-Like Alignment Tool (BLAT) is used to find genomic sequences that match a protein or DNA sequence. BLAT search results can be viewed on the University of California, Santa Cruz (UCSC) Genome Browser. This course demonstrates how to use BLAT to map a cDNA/mRNA sequence to a genome to identify exon-intron locations in the genomic sequence and a protein sequence to a genome to search for gene family members in the genome. It also demonstrates how to visualize the alignment in the UCSC genome browser and compare the results to a similar search done using NCBI’s BLAST.

Formats for Training:
Class
 
Class
 
       Dates Times Location Add to Calendar
       2013-03-12 01:30 PM-04:00 PM NIH Library Training Room, First Floor -- Registration Required 
      

Hands-on. Free. NIH and HHS staff only. NIH staff can register above. HHS staff can email a request to attend the class. Space is limited and registration is required.

       Objectives:
    Mapping of an mRNA/cDNA sequence to genomic sequence of the same species to identify exon-intron locations in the genomic sequence
    Mapping of a protein sequence to the genome to identify the coding regions in a genomic sequence and to search for gene family members in the same species
    Mapping of a protein sequence to the genome to find homologs in another species.
    Comparison of BLAT and NCBI's sequence similarity tool BLAST
       Who Should Attend:  
Interested NIH staff

 
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Last Updated: 6/27/2012 11:05 AM