Title |
Passaging Neural Stem Cells (Suspension Culture) |
Date Submitted |
May 5, 2012 |
Submitted by |
Efthymiou, Anastasia - anastasia.efthymiou@nih.gov |
Adapted from |
Gibco Protocol |
Contributors |
Efthymiou, Anastasia |
Affiliation(s) |
NIH CRM - NIAMS – Laboratory of Stem Cell Biology |
Early phase neurosphere formation (a) and high density neurosphere culture (b), phase contrast microscopy(1)
Neurospheres |
||
D-PBS without calcium and magnesium |
||
TrypLE Express |
||
StemPro NSC SFM complete medium |
||
StemPro NSC SFM Complete Media |
||
Component |
Final concentration |
Amount |
KnockOutTM D-MEM/F-12 |
1X |
97 mL |
GlutaMAXTM-I Supplement |
2 mM |
1 mL |
bFGF (prep as 100 μg/mL stock) |
20 ng/mL |
20 μL |
EGF (prep as 100 μg/mL stock) |
20 ng/mL |
20 μL |
StemPro® Neural Supplement |
2% |
2 mL |
(1)Laura Pacey KK, Shelley Stead, et al. Neural Stem Cell Culture: Neurosphere generation, microscopical analysis and cryopreservation. Protocol Exchange. (2006).
This page was last modified on October 23, 2012