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SS591 - Sequence Similarity Search: BLAST-Like Alignment Tool (BLAT)

Description:

    The BLAST-Like Alignment Tool (BLAT) is used to find genomic sequences that match a protein or DNA sequence. BLAT search results can be viewed on the University of California, Santa Cruz (UCSC) Genome Browser. This course demonstrates how to use BLAT to map a cDNA/mRNA sequence to a genome to identify exon-intron locations in the genomic sequence and a protein sequence to a genome to search for gene family members in the genome. It also demonstrates how to visualize the alignment in the UCSC genome browser and compare the results to a similar search done using NCBI?s BLAST.

Objectives:

  • Mapping of an mRNA/cDNA sequence to genomic sequence of the same species to identify exon-intron locations in the genomic sequence.
  • Mapping of a protein sequence to the genome to identify the coding regions in a genomic sequence and to search for gene family members in the same species.
  • Mapping of a protein sequence to the genome to find homologs in another species.
  • Comparison of BLAT and NCBI's sequence similarity tool BLAST.

Reasonable Accommodations/Interpreting Services

For deaf or hard-of-hearing individuals requiring interpreting services, please enter your request online in the Interpreting Services System at least five (5) days before the start of the class. If you have any questions regarding this service you may contact the ORS/DATS Interpreting Team at (301-4020-8180). Individuals who need other reasonable accommodations to participate in any of the CIT Training courses should let CIT Training know at least 5 days prior to the class.

Sections Available:

DateTimeLocationRegister for this Offering
Mar 12, 2013 Tuesday - 1:30pm - 4pmBuilding 10 - NIH Library Training Room (1st Floor)Register for Offering

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