Alliance of Glycobiologists

Svoboda M, Mann B, Goetz JA, Novotny MV. Examination of Glycan Profiles from IgG-Depleted Human Immunoglobulins Facilitated by Microscale Affinity Chromatography. Anal Chem. 2012; 84(7):3269-77.

Alley WR, Vasseur JA, Goetz JA, Svoboda M, Mann BF, Matei DE, Menning N, Hussein A, Mechref Y, Novotny MV. N-linked Glycan Structures and Their Expressions Change in the Blood Sera of Ovarian Cancer Patients. J Proteome Res. 2012; 11(4):2282-300.

Zhuang Z, Mitra I, Hussein A, Novotny MV, Mechref Y, Jacobson S. Microchip Electrophoresis of N-Glycans on Serpentine Separation Channels with Asymmetrically Tapered Turns. Electrophoresis 2011; 32(2):246-53.

Mann B, Madera M, Klouckova I, Dobrolecki LE, Hickey RJ, Hammoud ZT, Novotny MV. A Quantitative Investigation of Fucosylated Serum Glycoproteins with Application to Esophageal Adenocarcinoma. Electrophoresis 2010; 31, 1833-1841.

Alley WR Jr, Madera M, Mechref Y, Novotny MV. Chip-based reversed-phase liquid chromatography-mass spectrometry of permethylated N-linked glycans: a potential methodology for cancer-biomarker discovery. Anal Chem. 2010; 82(12):5095-106.

Alley WR, Novotny MV. Glycomic analysis of sialic acid linkages in glycans derived from blood serum glycoproteins. J Proteome Res. 2010; 9(6): 3062-72.

Mann B, Madera M, Klouckova I, Mechref Y, Dobrolecki LE, Hickey RJ, Hammoud ZT, Novotny MV. A quantitative investigation of fucosylated serum glycoproteins with application to esophageal adenocarcinoma. Electrophoresis. 2010; 31(11):1833-41.

Goetz JA, Mechref Y, Kang P, Jeng MH, Novotny, MV. Glycomic profiling of invasive and non-invasive breast cancer cells. Glycoconj J. 2009; 26(2):117-31.

Goldman R, Ressom HW, Varghese RS, Goldman L, Bascug G, Loffredo CA, Abdel-Hamid M, Gouda I, Ezzat S, Kyselova Z, Mechref Y, Novotny M.V. Detection of hepatocellular carcinoma using glycomic analysis. Clinical Cancer Res. 2009; 15(5):1808-13.

Mechref Y, Hussein A, Bekesova S, Pungpapong V, Zhang M, Dobrolecki LE, Hickey RJ, Hammoud ZT, Novotny MV. Quantitative serum glycomics of esophageal adenocarcinoma and other esophageal disease onsets. J Proteome Res. 2009; 8(6):2656-66.

Isailovic D, Kurulugama RT, Plasencia MD, Stokes ST, Kyselova Z, Goldman R, Mechref Y, Novotny MV, Clemmer DE. Profiling of human serum glycans associated with liver cancer and cirrhosis by IMS-MS. J Proteome Res. 2008; 7:1109-1117.

Kyselova Z, Mechref Y, Kang P, Goetz JA, Dobrolecki LE, Sledge GW, Schnaper L, Hickey RJ, Malkas LH, Novotny, MV. Breast cancer diagnosis and prognosis through quantitative measurements of serum glycan profiles. Clin Chem. 2008; 54:1166-1175.

Ressom HW, Varghese RS, Goldman L, An Y, Loffredo CA, Abdel-Hamid M, Kyselova Z, Mechref Y, Novotny M, Drake SK, Goldman R. Analysis of MALDI-TOF mass spectrometry data for discovery of peptide and glycan biomarkers of hepatocellular carcinoma. J Proteome Res. 2008; 7:603-610.

Kyselova Z, Mechref Y, Al Bataineh M, Dobrolecki L E, Hickey RJ, Sweeney CJ, Novotny MV. Alterations in the serum glycome due to metastatic prostate cancer. J Proteome Res. 2007; 6:1822-1832.

Zhuang Z, Starkey JA, Mechref Y, Novotny MV, Jacobson SC. Electrophoretic analysis of n-glycans on microfluidic devices. Anal Chem. 2007; 79:7170-7175.

Wu SL, Taylor AD, Lu Q, Hanash SM, Im H, Snyder M, Hancock WS. Identification of potential glycan cancer markers with sialic acid attached to sialic acid and up-regulated fucosylated galactose structures in epidermal growth factor receptor secreted from A431 cell line. Mol Cell Proteomics. 2013 Jan 31. [Epub ahead of print]

Zhao P, Nairn AV, Hester S, Moremen KW, O'Regan RM, Oprea G, Wells L, Pierce M, Abbott KL. Proteomic identification of glycosylphosphatidylinositol anchor-dependent membrane proteins elevated in breast carcinoma. J Biol Chem. 2012; 287(30):25230-40.

Mann BF, Goetz JA, House MG, Schmidt CM, Novotny MV. Glycomic and proteomic profiling of pancreatic cyst fluids identifies hyperfucosylated lactosamines on the N-linked glycans of overexpressed glycoproteins. Mol Cell Proteomics. 2012; 11(7):M111.015792.
Paik YK, Jeong SK, Omenn GS, Uhlen M, Hanash S, Cho SY, et al. A Chromosome-Centric Human Proteome Project to Characterize the Sets of Proteins Encoded in the Genome Nature Biotech., 2012; 30(3):221-3.

Rafalko A, Dai S, Hancock WS, Karger BL, Hincapie M. Development of a Chip/Chip/SRM Platform Using Digital Chip Isoelectric Focusing and LC-Chip Mass Spectrometry for Enrichment and Quantitation of Low Abundance Protein Biomarkers in Human Plasma. J Proteome Res. 2012; 11:808-17.

Tep S, Hincapie M, Hancock WS. A general approach for the purification and quantitative glycomic analysis of human plasma. Anal Bioanal Chem. 2012; 402:2687-700.

Zeng Z, Hincapie M, Pitteri SJ, Hanash SM, Schalkwijk J, Hogan JM, Wang H, Hancock W. A Proteomics Platform Combining Depletion, Multi-lectin Affinity Chromatography (M-LAC) and Isoelectric Focusing to Study the Breast Cancer Proteome. Anal Chem. 2011; 83:4845-54.

Wang D, Hincapie M, Rejtar T, Karger BL.? Ultrasensitive characterization of site-specific glycosylation of affinity purified haptoglobin from lung cancer patient plasma using 10 ?m i.d. porous layer open tubular (PLOT) LC-LTQ-CID/ETD-MS. Anal Chem. 2011; 83(6):2029-37.

Zeng Z, Hincapie M, Haab BB, Hanash S, Pitteri SJ, Kluck S, Hogan JM, Kennedy J, Hancock WS. The development of an integrated platform to identify breast cancer glycoproteome changes in human serum. J Chromatogr A. 2010 May 7; 1217(19):3307-15.

Abbott KL, Lim JM, Wells L, Benigno BB, McDonald JF, Pierce M. Identification of candidate biomarkers with cancer-specific glycosylation in the tissue and serum of endometrioid ovarian cancer patients by glycoproteomic analysis. Proteomics. 2010; 10(3): 470-81.

No publications on this topic were published during this year.

Abbott KL, Aoki K, Lim JM, Porterfield M, Johnson R, O'Regan RM, Wells L, Tiemeyer M, Pierce M. Targeted glycoproteomic identification of biomarkers for human breast carcinoma. J Proteome Res. 2008; 7(4):1470-80.

No publications on this topic were published during this year.

Kletter D, Singh S, Bern M, Haab BB. Global comparisons of lectin-glycan interactions using a database of analyzed glycan array data. Mol Cell Proteomics. 2013 Feb 11. [Epub ahead of print]

Padler-Karavani V, Song X, Yu H, Hurtado-Ziola N, Huang S, Muthana S, Chokhawala HA, Cheng J, Verhagen A, Langereis MA, Kleene R, Schachner M, de Groot RJ, Lasanajak Y, Matsuda H, Schwab R, Chen X, Smith DF, Cummings RD, Varki A. Cross-comparison of protein recognition of sialic acid diversity on two novel sialoglycan microarrays. J Biol Chem. 2012; 287(27):22593-608.

Bovin NV, Obukhova P, Shilova N, Rapoport E, Popova I, Navakouski M, Unverzagt C, Vuskovic M, Huflejt M. Repertoire of human natural anti-glycan immunoglobulins. Do we have auto-antibodies? Biochim Biophys Acta. 2012; 1820(9):1373-82.

Vuskovic MI, Fang H, Pass HI, Huflejt ME. Optimal combination of glycan-based serum diagnostic markers which maximize AUC. J Systemics Cybernetics Informatics. 2012; (In Press).

Zhou X, Zhang J, Wang D. Microwave-assisted method for fabrication of carbohydrate cluster microarrays on 3-dimensional hydrazide-dendrimer substrate. Methods Mol Biol. 2012; 808:401-11.

Jacob F, Goldstein DR, Bovin NV, Pochechueva T, Spengler M, Caduff R, Fink D, Vuskovic MI, Huflejt ME, Heinzelmann-Schwarz V. Serum anti-glycan antibody detection of non-mucinous ovarian cancers by using a printed glycan array. Int J Cancer. 2012; 130:138-46.

Pochechueva T, Jacob F, Goldstein DR, Huflejt ME, Chinarev A, Caduff R, Fink D, Hacker N, Bovin NV, Heinzelmann-Schwarz V. Comparison of printed glycan array, suspension array and ELISA in the detection of human anti-glycan antibodies. Glycoconj J. 2011; 28:507-17.

Vuskovic MI, Xu H, Bovin NV, Pass HI, Huflejt ME. Processing and analysis of serum antibody binding signals from Printed Glycan Arrays for diagnostic and prognostic applications. Int J Bioinf Res App. 2011; 7: 402-426.

Palma AS, Liu Y, Childs RA, Herbert C, Wang D, Chai W, Feizi T. The human epithelial carcinoma antigen recognized by monoclonal antibody AE3 is expressed on a sulfoglycolipid in addition to neoplastic mucins. Biochem Biophys Res Commun. 2011; 408:548-52.

Blixt O, Bueti D, Burford B, Allen D, Julien S, Hollingsworth M, Gammerman A, Fentiman I, Taylor-Papadimitriou J, Burchell JM. Autoantibodies to aberrantly glycosylated MUC1 in early stage breast cancer are associated with a better prognosis. Breast Cancer Res. 2011; 13(2):R25. [Epub ahead of print]

Kracun SK, Clo E, Clausen H, Levery SB, Jensen KJ, Blixt O. Random glycopeptide bead libraries for seromic biomarker discovery. J Proteome Res. 2010; 9(12):6705-14.

Blixt O, Clo E, Nudelman AS, S?rensen KK, Clausen T, Wandall HH, Livingston PO, Clausen H, Jensen KJ. A high-throughput O-glycopeptide discovery platform for seromic profiling. J Proteome Res. 2010; 9(10):5250-61. Erratum in: J Proteome Res. 2011 10(3):1436.

Dunlop DC, Bonomelli C, Mansab F, Vasiljevic S, Doores KJ, Wormald MR, Palma AS, Feizi T, Harvey DJ, Dwek RA, Crispin M, Scanlan CN. Polysaccharide mimicry of the epitope of the broadly neutralizing anti-HIV antibody, 2G12, induces enhanced antibody responses to self oligomannose glycans. Glycobiology. 2010; 20(7):812-23.

Taylor RE, Gregg CJ, Padler-Karavani V, Ghaderi D, Yu H, Huang S, Sorenson RU, Chen X, Inostroza J, Nizet V, Varki A. Novel mechanism for the generation of human xeno-autoantibodies against the nonhuman sialic acid N-glycolylneuraminic acid. J. Exp. Med. 2010; 207:1637-1646.

Wandall HH, Blixt O, Tarp MA, Pedersen JW, Bennett EP, Mandel U, Ragupathi G, Livingston PO, Hollingsworth MA, Taylor-Papadimitriou J, Burchell J, Clausen H. Cancer biomarkers defined by autoantibody signatures to aberrant O-glycopeptide epitopes. Cancer Res. 2010; 70(4):1306-13.

Carroll GT, Wang D, Turro NJ, Koberstein JT. Photogeneration of carbohydrate microarrays. In: ed. Dill K, Liu R, Grodzinski P. Microarrays: new development towards recognition of bio-signals. Springer, 2009.

Huflejt ME, Vuskovic MI, Vasiliu D, Xu H, Obukhova P, Shilova N, Tuzikov et al. Anti-carbohydrate antibodies of normal sera: findings, surprises and challenges. Mol Immunol. 2009; 46: 3037-3049.

Huang W, Wang D, Yamada M, Wang L. Chemoenzymatic synthesis and lectin array characterization of a class of N-glycan clusters. J Am Chem Soc 2009; 131(49):17963-71.

Zhou X, Turchi C, Wang D. Carbohydrate cluster microarrays fabricated on 3-dimensional dendrimeric platforms for functional glycomics exploration. J Proteome Res. 2009; 8(11): 5031-40.

Carroll GT, Wang D, Turro NJ, Koberstein JT. Photons to illuminate the universe of sugar diversity through bioarrays. Glycoconj J. 2008; 25(1):5-10. Epub 2007 Jul 4.

Shilova NV, Galanina OE, Rubina AY, Butvilovskaya VI, Huflejt ME, Chambers J, Roucoux A, Bovin NV. 2-Aminopyridine ? a label for bridging of oligo-saccharides HPLS profiling and glycoarray printing. Glycoconj J. 2008; 25: 11-14.

Wang D, Gedela S. Insights of new tools in glycomics research [editorial]. J Proteomics Bioinform. 2008; 1(8): 374-378.

No publications on this topic were published during this year.

Cao Z, Partyka K, McDonald M, Brouhard E, Hincapie M, Brand RE, Hancock WS, Haab BB. Modulation of glycan detection on specific glycoproteins by lectin multimerization. Anal Chem. 2013; 85(3):1689-98.

Lu Q, Padler-Karavani V, Yu H, Chen X, Wu SL, Varki A, Hancock WS. LC-MS Analysis of Polyclonal Human Anti-Neu5Gc Xeno-Autoantibodies Immunoglobulin G Subclass and Partial Sequence Using Multistep Intravenous Immunoglobulin Affinity Purification and Multienzymatic Digestion. Anal Chem. 2012; 84(6):2761-8.

Pejchal R, Doores KJ, Walker LM, Khayat R, Huang PS, Wang SK, Stanfield RL, Julien JP, Ramos A, Crispin M, Depetris R, Katpally U, Marozsan A, Cupo A, Maloveste S, Liu Y, McBride R, Ito Y, Sanders RW, Ogohara C, Paulson JC, Feizi T, Scanlan CN, Wong CH, Moore JP, Olson WC, Ward AB, Poignard P, Schief WR, Burton DR, Wilson IA. A Potent and Broad Neutralizing Antibody Recognizes and Penetrates the HIV Glycan Shield. Science 2011; 334:1097-103.

Abbott KL, Pierce JM. Lectin-based glycoproteomic techniques for the enrichment and identification of potential biomarkers. Methods Enzymol. 2010; 480:461-76.

Kullolli M, Hancock WS, Hincapie M. An automated platform for fractionation of human plasma glycoproteome in clinical proteomics. Anal Chem. 2010; 82(1):115-20.

Diaz SL, Padler-Karavani V, Ghaderi D, Hurtado-Ziola N, Yu H, Brinkman-Van der Linden ECM, Varki A, Varki N.M. Sensitive and specific detection of the non-human sialic acid n-glycolylneuraminic acid in human tissues and biotherapeutic products. PLoS ONE. 2009; 4(1):e4241. Epub 2009 Jan 21.

Yue T, Goldstein IJ, Hollingsworth MA, Kaul K, Brand RE, Haab BB. The prevalence and nature of glycan alterations on specific proteins in pancreatic cancer patients revealed using antibody-lectin sandwich arrays. Mol Cell Proteomics. 2009; 8(7):1697-707. Epub 2009 Apr 17.

Kullolli M, Hancock WS, Hincapie M. Preparation of a high-performance multi-lectin affinity chromatography (HP-M-LAC) adsorbent for the analysis of human plasma glycoproteins. J Sep Sci. 2008; 14, 2733-9.

Ralin DW, Dultz SC, Silver JE, Kullolli M, Hancock WS, Hincapie M. Kinetic analysis of glycoprotein-lectin interactions by label free internal reflection. Clin Proteomics. 2008;4,37-46.

Wang D, Wu A. Carbohydrate microarrays for lectin characterization and glyco-epitope identification. In: Nilsson, CL, ed. Lectins: Analytical Technologies. Elsevier; 2007.

Guo H, Nairn A, Dela Rosa M, Nagy T, Zhao S, Moremen K, Pierce M. Transcriptional regulation of the protocadherin cluster during Her-2 protein-induced mammary tumorigenesis results from altered N-glycan branching. J Biol Chem. 2012; 287(30):24941-54.

Langer MD, Guo H, Shashikanth N, Pierce JM, Leckband DE. N-glycosylation alters cadherin-mediated intercellular binding kinetics. J Cell Sci. 2012; 125(Pt 10):2478-85.

Human xeno-autoantibodies against a non-human sialic acid serve as novel serum biomarkers and immunotherapeutics in cancer. Cancer Res. 2011; 71:3352-3363

Guo HB, Johnson H, Randolph M, Nagy T, Blalock R, Pierce M. Specific posttranslational modification regulates early events in mammary carcinoma formation. Proc Natl Acad Sci U. S. A. 2010; 107(49):21116-21

No publications on this topic were published during this year.

Abbott KL, Nairn AV, Hall EM, Horton MB, McDonald JF, Moremen KW, Dinulescu DM, Pierce M. Focused glycomic analysis of the N-linked glycan biosynthetic pathway in ovarian cancer. Proteomics. 2008; 8(16): p. 3210-20.

No publications on this topic were published during this year.

No publications on this topic have yet been published this year.

SIGLEC12, a Human-specific Segregating (Pseudo)gene, Encodes a Signaling Molecule Expressed in Prostate Carcinomas. Mitra N, Banda K, Altheide TK, Schaffer L, Johnson-Pais TL, Beuten J, Leach RJ, Angata T, Varki N, Varki A. J Biol Chem. 2011; 286:23003-11.

No publications on this topic were published during this year.

Thomas E, Newsom-Davis TE, Wang D, Steinman L, Chen PFT, Wang L, Simon AK, Screaton, GR. Enhanced immune recognition of cryptic glycan markers in human tumors. Cancer Res. 2009;69 (5):2018-2025.

Costa NR, Mendes N, Marcos NT, Reis CA, Caffrey T, Hollingsworth MA, Santos-Silva F. Relevance of MUC1 mucin variable number of tandem repeats polymorphism in H pylori adhesion to gastric epithelial cells. World J Gastroenterol. 2008;14(9):1411-4.

Hedlund M, Padler-Karavani V, Varki N, Varki A. Evidence for a human-specific mechanism for diet and antibody-mediated inflammation in carcinoma progression. Proc Natl Acad Sci. 2008;105:18936-18941.

Padler-Karavani V, Yu H, Cao H, Chokhawala H, Karp F, Varki N, Chen X, Varki A. Diversity in specificity, abundance and composition of anti-neu5Gc antibodies in normal humans: potential implications for disease. Glycobiology. 2008;18:818-830.

Swanson BJ, McDermott KM, Singh PK, Eggers JP, Crocker PR, Hollingsworth MA. MUC1 is a counter-receptor for myelin-associated glycoprotein (siglec-4a) and their interaction contributes to adhesion in pancreatic cancer perineural invasion. Cancer Res. 2007;67(21):10222-9.

Wade A, Robinson AE, Engler JR, Petritsch C, David James C, Phillips JJ. Proteoglycans and their roles in brain cancer. FEBS J. 2013; In Press.

No reviews were published during this year.

Padler-Karavani V, Varki A. Potential impact of the non-human sialic acid N-Glycolylneuraminic acid on transplant rejection risk. Xenotransplantation (Invited Commentary), 2011; 18:1-5.

Wang D. (2011). Carbohydrate Antigen Microarrays. In: ed. Chevolot Y. Carbohydrate Microarrays, Microarray Methods & Protocols, Humana Press, 2011; pp. 241-249.

Carroll GT, Wang D. Photoimmobilization of Saccharides, In: ed. Chevolot Y. Carbohydrate Microarrays, Microarray Methods & Protocols, Humana Press, 2011; (in press).

Tousi F, Hancock WS, Hincapie, M.? Technologies and strategies for glycoproteomics and glycomics and their application to clinical biomarker research. Anal Methods. 2011; 3(1):20?32.

Chen X, Varki A. Advances in the biology and chemistry of sialic acids. ACS Chem Biol. 2010;5(2):163-76.

Taylor AD, Hancock WS, Hincapie M, Taniguchi N, Hanash SM. Towards an integrated proteomic and glycomic approach to finding cancer biomarkers. Genome Med. 2009;1(6):57.

Wang D. Carbohydrate arrays. In: Matson, RS, ed. Microarray Methods & Protocols. 2008;CRC Press.

Liu Y, Palma AS , Feizi T. Carbohydrate microarrays: key developments in glycobiology. Biol Chem 2009;390:647-656.

Zhou X, Carroll GT, Turchi C, Wang D. Carbohydrate microarrays as essential tools of postgenomic medicine, In: Garg HG, Cowman MK, Hales CA, ed. Carbohydrate chemistry and biology for physicians. Elsevier, 2008.

No reviews were published during this year.