Completed OCG Programs

NIH Mammalian Gene Collection (MGC)

The goal of the Mammalian Gene Collection (MGC), a trans-NIH initiative, was to provide full-length open reading frame (FL-ORF) clones for human, mouse, cow, and rat genes. The MGC has created "expression-ready" clones for all Homo sapiens genes, which can be used by the research community for expression of proteins for large-scale proteomic analyses.

In 2009, the final publication of the MGC Project Team marked the successful completion of the MGC, which includes 17,502 human genes and 17,702 mouse genes. Together, 73,000 FL-ORFs are available, representing more than 50,000 unique genes from human, mouse, rat, and cow. To learn more about the Mammalian Gene Collection (MGC), visit http://mgc.nci.nih.gov/.

MGC is part of the ORFeome Collaboration (OC), which was formed to provide the research community with sequence-validated, full-ORF human cDNA clones in the Gateway® vector format, allowing easy transfer of the ORF sequences into nearly any type of expression vector. Details on the OC clones and OC distributors are given at: http://www.orfeomecollaboration.org/

MGC infrastructure and protocols are also being applied to two other gene collection projects:

The XGC and ZGC are NIH initiatives that support the production of cDNA libraries, clones, and sequences to provide a set of full-length (open reading frame) sequences and cDNA clones of expressed genes for Xenopus (both X. laevis and X. tropicalis) for the XGC and zebrafish for the ZGC. All resources generated by the XGC are publicly accessible to the biomedical research community.

To learn more about the Mammalian Gene Collection, click here.