Industry Circular

Number: 60-3

Date: March 1, 1960

Office of the Commissioner of Internal Revenue Alcohol and Tobacco Tax Division

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MATERIALS AUTHORIZED FOR TREATMENT OF WINES

Proprietors of bonded wine cellars and others concerned:

Purpose. This circular is to advise you that the Food and Drug Administration, Department of Health, Education, and Welfare, under the Food Additives Amendment of 1958, has approved certain pectolytic enzymes, dervied from Aspergillus niger, produced in accordance with good manufacturing pro- cedure, for use in the production of wine on and after March 5, 1960; and has advised that polyvinylpyrrolidone may be used in wine production until further notice.

Background. Industry Circular No. 59-52, dated July 28, 1959, and Industry Circular No. 59-52, Supplement No. 1, dated August 25, 1959, advised you that polyvinylpyrrolidone (known as PVP) and certain enzymes (pectinol and pectizyme [pectinase]) were not subject to the prohibited use section of the Food Additives Amendment of 1958, until March 5, 1960, and, therefore, their use could be con- tinued until March 5, 1960. You were also advised that studies on their safety were in progress.

Materials Authorized. The following materials are considered safe and may be used, as specified herein, on and after March 5, 1960:

Material Use Limitation

Pectolytic Enzymes: Pectinol 59L To clarify wine. 0.3 pound per 1000 gallons of wine.

Klerzyme 200 To clarify wine. One half pound per 1000 gallons of wine.

Pectinol 100D To clarify wine. 1 pound per 1000 gallons Pectinol 10M of wine. Pectinol R10

Pectinase To clarify wine. 10 pounds per 1000 gallons Concentrate of wine. Pectinol A Pectinol M Pectinol 0 Pectinase Regular Material Use Limitation Pectinase D Regular Pectolase

Polyvinylpyrrolidone To clarify and The wine after treatment must stabilize wine. contain less than one-hun- dredth of one percent (0.01%) of polyvinylpyrrolidone.§ 240.524 A proprietor desiring to use any pectolytic enzyme not specified in this circular must, prior to such use, file a notice and obtain approval therefor in accordance with regulations in 26 CFR 240.530. The notice shall state the maxi- mum quantity of the enzyme to be used per 1,000 gallons of wine; and that the manufacturer of the enzyme guarantees it to be safe and that it was derived from Aspergillus niger and manufactured in accordance with good commercial pro- cedure. A four (4) ounce sample of the enzyme must be submitted with the notice.

Inquiries. Inquiries regarding this circular should refer to its number and should be addressed to the office of your assistance regional commissioner (alcohol and tobacco tax).

Dwight E. Avis

Director, Alcohol and Tobacco Tax Division

IRS - D. C. - 58213