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Analysis Results

The Stem Cell Unit has assessed these cell lines and will be comparing other pluripotent human cells to this database of information. Gene expression data for selected lines can be found on the NINDS Stem Cell Data Management System.

RT-PCR test results, growth curves, and subcloning test results are also available for selected lines.

Click on any linked test title below for detailed analysis results.

BG01 BG02 BG03 ES01 ES02 ES03 ES04 ES05 ES06 SA01 SA02
Mycoplasma Testing1 - ND ND - - - - ND - ND ND
Viral Testing2 - - - - - - - ND - ND ND
Genotyping Analysis + + + + + + + + + + +
Karyotyping Analysis + + + + + + + + + + +
Immunostaining
Oct-4 ND + + + ND + ND + + ND ND
SSEA-1 ND - -/+ -/+ ND - ND - - ND ND
SSEA-4 ND + + + ND + ND ND ND ND ND
Tra-1-81 ND + + + ND + ND + + ND ND
Alkaline Phosphatase ND ND ND + ND ND ND + + + +
FACS
SSEA-1 - - - -/+ - -/+ - - - - -/+
SSEA-4 + + + + +/- + +/- + + + +
TRA-1-60 ND ND ND + ND ND ND ND ND ND ND
TRA-1-81 + + + + + + + + + + +
Oct-4 + + + + + + + + + + +
HLA Typing3 + + + + + + + + + + +

ND = Not done/determined

TE03 TE04 TE06 UC01 UC06 WA01 WA07 WA09 WA13b WA14
Mycoplasma Testing1 - ND - ND - - - - ND ND
Viral Testing2 - ND - - - - - - - -
Genotyping Analysis + + + + + + + + + +
Karyotyping Analysis + + + + + + + + + +
Immunostaining
Oct-4 + ND + ND + + + + + +
SSEA-1 -/+ ND -/+ ND -/+ - - - - -/+
SSEA-4 + ND + ND + + + + + +
Tra-1-81 + ND + ND + + + + + +
Alkaline Phosphatase ND + + ND + + + + ND ND
FACS
SSEA-1 - -/+ -/+ - -/+ - -/+ - - -
SSEA-4 + + + + + + + + + +
TRA-1-60 ND ND + ND + + + + + +
TRA-1-81 + + + + + + + + + +
Oct-4 + + + + + + + + + +
HLA Typing3 + ND + + + + + + + +

ND = Not done/determined

1. Both PCR and direct culture. Those cell lines tested have been shown to be negative. (Tests performed by ATCC.)

2. Hepatitis B, Hepatitis C, HIV-1, HIV-2, HTLV-I/II, HSV1, HSV2, EBV, and CMV. Those cell lines tested have been shown to be negative. (Tests performed by GIVF Laboratories.)

3. Tests performed by Invitrogen.