Title |
Cryopreserving Neural Stem Cells |
Date Submitted |
May 5, 2012 |
Submitted by - |
Efthymiou, Anastasia - anastasia.efthymiou@nih.gov |
Adapted from - |
Gibco Protocol |
Contributors - |
Efthymiou, Anastasia |
Affiliation(s) - |
NIH CRM - NIAMS – Laboratory of Stem Cell Biology |
Neural Stem Cells |
||
KnockOut DMEM/F-12 |
||
StemPro NSC SFM |
||
FGF basic, Recombinant Human (bFGF) |
||
EGF, Recombinant Human | ||
TrypLE Select (1X) | ||
D-PBS | ||
DMSO | ||
StemPro NSC SFM complete medium |
||
Component |
Final concentration |
Amount |
KnockOutTM D-MEM/F-12 |
1X |
48.5 mL |
GlutaMAXTM-I Supplement |
2 mM |
0.5 mL |
bFGF |
20 ng/mL |
1 μL |
EGF |
20 ng/mL |
1 μL |
StemPro® Neural Supplement |
2% |
1 mL |
Freezing medium | ||
Component |
Final concentration |
Amount |
StemPro NSC SFM CM | 90% | 9mL |
without bFGF and EGF | ||
DMSO | 10% |
This page was last modified on October 18, 2012