National Cancer Institute U.S. National Institutes of Health www.cancer.gov
Cancer Diagnosis Program (CDP)
CDP Tissue Microarrays

CDP Breast Cancer Prognostic TMA (Case Sets 9-19)

Stage I TMA

The CDP 2008 Series Stage I Prognostic TMA represents a collection of 590 stage I invasive breast cancer tissue specimens with associated clinico-pathologic data and clinical follow-up information. These cases are divided into five non-overlapping case sets. The case sets are labeled case sets 9-13 to distinguish them from case sets used on previous TMAs. Each case set is arrayed in quadruplicate, producing a total of 20 TMA blocks. Table 1 below gives the distribution of breast tissue and control samples across the 5 case sets. The number of cores represented on any single TMA section is 146 (126 breast tissue cores + 20 control cores). Normal breast epithelium cores will be 1.0 mm and all other cores will be 0.6 mm in diameter. An applicant whose request for the Stage I Prognostic TMA is approved would be required to accept TMAs from all five case sets, with a minimum of two sections (from different replicate blocks) from each case set. Thus, the minimum number of TMA sections that any investigator would receive is ten (5 case sets × 2 replicate TMA blocks per case set × 1 section per TMA block). Click Here for the Excel map of sections[XLS] or Text alternative for map of sections.

Table 1. Specimens represented in the CDP 2008 Series Stage I Prognostic TMA case sets. Each case set is used to produce quadruplicate TMA blocks, so numbers presented in table should be multiplied by four to obtain the number of cores required for the full Stage I TMA set.
Type of Case Case
Set 9
Case
Set 10
Case
Set 11
Case
Set 12
Case
Set 13
Total
Stage I invasive breast carcinoma 118 118 118 118 118 590
Normal breast epithelium 5 5 5 5 5 25
Breast fibroadenoma 3 3 3 3 3 15
Total distinct breast tissue specimens 126 126 126 126 126 630
 
Cell line controls            
MCF-7 2 2 2 2 2 10
MCF-10A 2 2 2 2 2 10
SK-BR-3 2 2 2 2 2 10
SK-OV-3 2 2 2 2 2 10
T-47D 2 2 2 2 2 10
Normal non-breast tissue controls            
Salivary gland 2 2 2 2 2 10
Endometrium 2 2 2 2 2 10
Colon 2 2 2 2 2 10
Tonsil 2 2 2 2 2 10
Prostate 2 2 2 2 2 10
Total control cores per replicate TMA block 20 20 20 20 20 100
 
Grand total cores per replicate TMA block 146 146 146 146 146 730

Stage II TMA

The CDP 2008 Series Stage II Prognostic TMA represents a collection of 398 stage II invasive breast cancer tissue specimens with associated clinico-pathologic data and clinical follow-up information. These cases are divided into four non-overlapping case sets. The case sets are labeled case sets 14-17 to distinguish them from case sets used on previous TMAs. Each case set is arrayed in quadruplicate, producing a total of 16 TMA blocks. Table 2 below gives the distribution of breast tissue and control samples across the 4 case sets. The number of cores represented on any single TMA section is 127-128 (107-108 breast tissue cores + 20 control cores). Normal breast epithelium cores will be 1.0 mm and all other cores will be 0.6 mm in diameter. An applicant whose request for the Stage II Prognostic TMA is approved would be required to accept TMAs from all four case sets, with a minimum of two sections (from different replicate blocks) from each case set. Thus, the minimum number of TMA sections that any investigator would receive is eight (4 case sets × 2 replicate TMA blocks per case set × 1 section per TMA block). Click Here for the Excel map of sections[XLS] or Text alternative for map of sections.

Table 2. Specimens represented in the CDP 2008 Series Stage II Prognostic TMA case sets. Each case set is used to produce quadruplicate TMA blocks, so numbers presented in table should be multiplied by four to obtain the number of cores required for the full Stage II TMA set.
Type of Case Case
Set 14
Case
Set 15
Case
Set 16
Case
Set 17
Total
Stage II invasive breast carcinoma 99 99 100 100 398
Normal breast epithelium 5 5 5 5 20
Breast fibroadenoma 3 3 3 3 12
Total distinct breast tissue specimens 107 107 108 108 430
 
Cell line controls          
MCF-7 2 2 2 2 8
MCF-10A 2 2 2 2 8
SK-BR-3 2 2 2 2 8
SK-OV-3 2 2 2 2 8
T-47D 2 2 2 2 8
Normal non-breast tissue controls          
Salivary gland 2 2 2 2 8
Endometrium 2 2 2 2 8
Colon 2 2 2 2 8
Tonsil 2 2 2 2 8
Prostate 2 2 2 2 8
Total control cores per replicate TMA block 20 20 20 20 80
 
Grand total cores per replicate TMA block 127 127 128 128 510

Stage III TMA

The CDP 2008 Series Stage III Prognostic TMA represents a collection of 181 stage III invasive breast cancer tissue specimens with associated clinico-pathologic data and clinical follow-up information. These cases are divided into two non-overlapping case sets. The case sets are labeled case sets 18-19 to distinguish them from case sets used on previous TMAs. Each case set is arrayed in quadruplicate, producing a total of 8 TMA blocks. Table 3 below gives the distribution of breast tissue and control samples across the 2 case sets. The number of cores represented on any single TMA section is 118 or 119 (98 or 99 breast tissue cores + 20 control cores). Normal breast epithelium cores will be 1.0 mm and all other cores will be 0.6 mm in diameter. An applicant whose request for the Stage III Prognostic TMA is approved would be required to accept TMAs from both case sets, with a minimum of two sections (from different replicate blocks) from each case set. Thus, the minimum number of TMA sections that any investigator would receive is four (2 case sets × 2 replicate TMA blocks per case set × 1 section per TMA block). Click Here for the Excel map of sections[XLS] or Text alternative for map of sections.

Table 3. Specimens represented in the CDP 2008 Series Stage III Prognostic TMA case sets. Each case set is used to produce quadruplicate TMA blocks, so numbers presented in table should be multiplied by four to obtain the number of cores required for the full Stage III TMA set.
Type of Case Case
Set 18
Case
Set 19
Total
Stage III invasive breast carcinoma 91 90 181
Normal breast epithelium 5 5 10
Breast fibroadenoma 3 3 6
Total distinct breast tissue specimens 99 98 197
 
Cell line controls      
MCF-7 2 2 4
MCF-10A 2 2 4
SK-BR-3 2 2 4
SK-OV-3 2 2 4
T-47D 2 2 4
Normal non-breast tissue controls      
Salivary gland 2 2 4
Endometrium 2 2 4
Colon 2 2 4
Tonsil 2 2 4
Prostate 2 2 4
Total control cores per replicate TMA block 20 20 40
 
Grand total cores per replicate TMA block 119 118 237

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