Scanning Probe Microscopy

Expertise:

• Sub-nm resolution imaging of molecular complexes, supported lipid bilayers, cells, and tissues
• Molecular recognition, protein unfolding
• Force spectroscopy, elastic properties
• Simultaneous, co-localized AFM and fluorescence (epi- and TIRF) microscopy
• Mathematical modeling, finite element analysis

Emilios Dimitriadis, Ph.D., Chief
(301) 435-1952
dimitre@mail.nih.gov

    Infrared Imaging and Thermometry

Expertise:

• Intraoperative and bedside imaging
• Functional optical imaging
• Multi-spectral imaging
• In vivo optical imaging to guide functional tests, targeted drug delivery, and ex vivo organ resuscitation 
• Remote sensing for clinical research using cellular phone technology 
• Image processing and data mining

Alexander M. Gorbach, Ph.D., Chief
(301) 496-7677
agorbach@mail.nih.gov

    Quantitative Methods for Macromolecular Interactions

Expertise:

• Characterization of individual macromolecules
• Characterization of interactions of macromolecules (self- and hetero association) and small ligands

Patrick Brown, Ph.D., Chief
(301) 435-9371
brownpatr@mail.nih.gov

    Micro Analytical Immunochemistry

Expertise:

• Micro-immunoaffinity capillary electrophoresis to quantify analyte concentrations in biological samples
• Development of novel immunoassays for microliter and sub-microliter biological fluid and tissue samples
• Multianalyte ELISA arrays using Quansys^TM imaging system
• Characterization of biological molecule interactions using Surface Plasmon Resonance (SPR)

Heather Kalish, Ph.D., Chief
(301) 435-5498
kalishh@mail.nih.gov

    Microfabrication and Microfluidics

Expertise:

• Design, fabrication and implementation of microfluidic devices
• Rapid turnaround of single-layer templates down to ~10um dimensions
• Structured surface modification, including microcontact printing

Nicole Morgan, Ph.D., Chief
(301) 435-1947
morgann@mail.nih.gov

    Electron Microscopy

Expertise:

• Transmission electron microscopy
• Scanning electron microscopy
• Specimen preparation, including fixation by rapid freezing
• Immuno EM
• Electron Tomography
• Cryo EM

Guofeng Zhang, Ph.D., Chief
(301) 451-3856
zhangguo@mail.nih.gov

Biomedical Instrumentation and Computation

Instrumentation Expertise:

• Electronic design and virtual instrumentation for basic and clinical research
• Signal acquisition and processing 
• Additions/modifications to commercial systems

Computational Expertise:

• Analysis of data content of DNA, RNA and protein sequence segments and their relationship to structure and function
• DNA/RNA splice site analysis using information theory and measures
• Statistical analysis of noisy data sets
• Investigations of explanations for subtle differences among similar data groups
• Conversion of data in various formats (e.g., EXCEL to Unix)

For more information, contact:

Henry S. Eden, M.D., Ph.D.
(301) 435-1953
edenh@mail.nih.gov