Sequence Analysis: Making Sense of DNA and Protein Sequences
Description:
In this class, students will find a gene within a eukaryotic DNA sequence. They will then learn how to predict the function of the implied protein product by seeking sequence similarities to proteins of documented function using BLAST and other tools. Finally, students will find a 3D modeling template for this protein sequence using a Conserved Domain Database Search.
During the first hour, the instructor will walk students through an analysis of an uncharacterized genomic sequence from a GenBank record. During the second hour of the class, students will perform the same analysis on another genomic sequence.
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Formats for Training:
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Class
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Dates |
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Hands-on. Free. NIH and HHS staff only. NIH staff can register above. HHS staff can email a request to attend the class. Space is limited and registration is required.
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Objectives:
- Exon prediction and the generation of a protein sequence
- Protein function prediction
- Identification of the characteristic domains and motifs in the protein
- Mapping of the protein sequence onto the structure of a protein with similar sequence
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Benefits:
This course will provide an introduction to protein sequence analysis, beginning with the prediction of a gene within a DNA sequence and ending with a model 3-D protein structure.
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Who Should Attend:
Any NIH staff member with an interest in the topic
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Useful Handouts/Links:
This class is based on one of the mini-courses developed by the instructor Dr. Medha Bhagwat while working at NCBI
NCBI Mini-Courses
On-line tutorials from Open Helix:
The NIH Library has obtained a subscription for over one hundred online tutorials developed by Open Helix on several bioinformatics databases and resources for the entire NIH staff provides . Registration is required to access the tutorials which are not offered free by Open Helix. The list of the tutorials and the link to the registration can be accessed from
the Open Helix page.
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