DNA Microarray Flowchart
Steps in microarray screening
Core services shown in green; user tasks shown in blue
1. Consultation on experimental design
- Experimental objective
- Number of samples required
- Amount of sample required
- Protocol for sample isolation
- Type of GeneChip required
|
5. Sample analysis
- Quantification
- Quality control by BioAnalyzer
- User will be consulted if samples do not meet lab standards
|
2. Submission of experimental worksheet
- Download MIAME form
- Complete Project, Experiment,
User, and RNA Sample pages
- Submit form to George Poy
|
6. Microarray screening
- Sample labeling
- Hybridization
- Signal quantification
|
3. Scheduling
- Order GeneChips
- Schedule run
|
7. Data quality assessment
- Statistical analysis to generate
QC metrics and outlier detection
- Summary report and raw data
available via EDGE web interface
|
4. Preparation of samples
- Isolate RNA or DNA
- Deliver samples and GeneChips
to Core lab (Bldg 8, Rm 1A11)
three days prior to run date
|
8. Data analysis
- Bioinformatic tools Partek Pro
and GeneSpring available to user
- Additional bioinformatic support
available on a collaborative basis;
contact Core staff if interested
|
* Documents in Excel format require the free Excel Viewer for viewing. 
Page last updated: May 31, 2009
