3-DEAZAURIDINE

NSC - 126849

SMILE String: O1[CH](N2C(=O)CC(=O)C=C2)[CH]([CH]([CH]1CO)O)O

Chemical Name: 1-13- D- ribofuranosy1- 2,4(1H,3H)- pyridinedione CAS Registry Number: 39935 -49 -4

CASE Registry Number: 39935-49-4

Molecular Formula: C10H13NO6    M.W.: 243.2

Approximate Solubility:         (mg/mL)

H2O                                18
DMSO                            > 150
EtOH                            < 1
DMF                             > 150
EtOAc                             0.2
CHC13                             0.2

Stability:

Bulk: A sample stored at 60 °C for 30 days showed no decomposition by UV absorption or paper chromatography.

Solution: A 2% aqueous solution held at room temperature for 24 hours showed no decomposition by UV absorption or paper chromatography.

Ultraviolet Absorption:

(0.1 N NaOH)

max = 255 ± 2 nm               E = 7,850 - 8,200

High Performance Liquid Chromatography:

Column: Altex Ultrasphere, 5u, 250 x 4.6 mm i.d.

Mobile Phase: Methanol/0.1 M acetate buffer, pH 4 (3:97)


Flow Rate: 1 mL/min

Detection: UV at 254 nm

Sample Preparation: 0.2 mg/mL in mobile phase

Internal Standard: Thymidine, 0.06 mg/mL in mobile phase

Retention Volume: 9.6 mL (NSC - 126849)
                  18.7 mL (I.S)

Optical Rotation:

(c = 1, H2O)            [a]20D = 43 ± 2°