THYMIDINE

NSC - 21548

SMILE String: O=c1n(cc(c(=O)[nH]1)C)[CH]1O[CH]([CH](C1)O)CO

Chemical Name: 1-(2-Deoxy-(3-D-ribofuranosyl)-5-methyl-2,4(1H,3H)-pyrimidinedione

CAS Registry Number: 50-89-5

Molecular Formula: C10H14N2O5          M.W.: 242.2

Approximate Solubility:          (mg/mL)

Water                               50

Stability:

Solution: A 1% solution of thymidine in water, kept at room temperature, is stable for at least 24 hours (HPLC).

Ultraviolet Absorption:

(0.1 N HC1)

ma = 267 ± 2 nm          E = 9,700 - 9,900

High Performance Liquid Chromatography:

Column: Alltech Spherisorb-ODS-1, 5u, 25 x 0.46 cm

Mobile Phase: 7% acetonitrile in 0.05M KH

Flow Rate: 1.0 mL/min

Detection: UV at 255

Sample Preparation: approximately 1 mg sample in 1.0 mL methanol/water or 2.0 mL internal standard solution

Internal Standard: Adenosine (0.5 mg/mL in water)

Retention Volume: 8.5 mL (NSC - 21548)
                  5.9 mL (I.S.)

Optical Rotation:

(c = 1, H2O)     [a]25 D = +18 ± 0.5°

Toxicity Data:

Mouse(ip): LD50 2512 mg/kg
Journal of Pharmacology & Experimental Therapeutics, 207,504 (1978)