JB -11

NSC - 249008

SMILE String: n1c(nc(c2c1ccc(c2C)CNc1cc(c(c(c1)OC)OC)OC)N)N

Chemical Name: 5-Methyl-6-[[(3,4,5- trimethoxyphenyl)amino]methyl]-2,4-quinazolinediamine, acetate

CAS Registry Number: 52128-35-5

Molecular Formula: C19H23N5O3        M.W.:429.5

Approximate Solubility:             (mg/mL)
Water                                1 - 2
pH 4 Acetate buffer                    < 1
pH 9 Acetate buffer                    < 1
0.1NHC1                              1 - 5
0.1 N NaOH                             < 1
Methanol                           12 - 15
95% Ethanol                          1 - 5
10% Ethanol                          1 - 3
Dimethylacetamide                  10 - 15
Dimethylsulfoxide         approimately 100

Stability:
 Bulk: A sample stored at 60 °C for 30 days showed no decomposition (UV, HPLC).

 Solution: A solution in 5% methanol (0.5 mg/mL) showed no decomposition after 9 days (UV). A solution in 5%
dimethylacetamide /pH 4 acetate buffer showed no decomposition after 48 hours(HPLC).

Ultraviolet Absorption:

(0.1 N NaOH)

  max                          E
237 ±2nm                46,970 - 47,922
341 ±2nm                 4,410 - 4,680

High Performance Liquid Chromatography:

 Column: pondapak C 300 x 3.9 mm i.d.

 Mobile Phase: CH3CN/3% acetic acid in water, 20/80, v/v

 Flow Rate: 0.8 mL/min

 Detection: UV at 254 nm

 Sample Preparation: 0.5 mg/mL in methanol containing internal standard

 Internal Standard: 0.35 mL/mL acetophenone in methanol

 Retention Volume: 6.4 mL (NSC-249008)
                   9.1 mL (I.S.)