MITINDOMIDE

NSC - 284356

SMILE String: O=C1[CH]2[CH](C(=O)N1)[CH]1C=C[CH]2[CH]2[CH]1[CH]1C(=O)NC(=O)[CH]21

Chemical Name: Octahydro-4,8-ethenopyrrolo[3',4':3,4]cyclobut[1,2- f]isoindole-1,3,5,7-(2H,611)-tetrone

CAS Registry Number: 10403-51-7

Molecular Formula: C14H12N204             M.W.: 272.3

Approximate Solubility:                                           (mg/mL)
p- dioxane                                               slightly soluble
Aqueous NaOH (2 moles NaOH per mole of compound)                     > 20

Stability:

 Solution: A solution was prepared by dissolving 100 mg of drug in 3.0 mL of 0.25 N NaOH. This solution was diluted to 5.0 mL with water to give a final concentration of 20 mg/mL. At room temperature this solution shows 10% decomposition in 40 minutes and 50% decomposition in 5 hours (HPLC).

Ultraviolet Absorption:

(p-dioxane)

max = 252 ± 2 nm                  E = 346 - 366

High Performance Liquid Chromatography:

 Column: 300 mm x 4.6 mm i.d. RP-8

 Mobile Phase: 10% CH in H pH adjusted to 4 with acetic acid

 Flow Rate: 1.0 mL/min

 Detection: UV at 254 nm

 Sample Preparation: A 3.4 -mg sampling is quickly dissolved in 1.0 mL of 0.05 N NaOH with the aid of sonication and vigorous mixing. At exactly 1.0 min after addition of the solvent the solution is chromatographed.

Retention Volume: 8.1 mL (NSC-284356)

Toxicity Data:

Mouse(iv): LD50: 193 mg/kg
 NCI Screening Program Data Summary