MITOGUAZONE

NSC - 32946

SMILE String: C(N)(=N)N/N=C/C(=N/NC(N)=N)C.Cl

Chemical Name: 2,2'-(1-Methyl-1,2-ethanediylidene)bis(hydrazinecarboximidamide),dihdrochloride, monohydrate

Other Names: Methyl GAG; Methylglyoxal Bisguanylhydrazone Dihydrochloride

CAS Registry Number: 7059-23-6

Molecular Formula: C5H12N8.2HCl         M.W: 275.1

Approximate Solubility:            (mg/mL)

0.1 N NaOH                         soluble
Water                                > 100
0.01 N NaOH                 partly soluble
0.1 N HC1                   partly soluble
MeOH                        partly soluble
50% EtOH                    partly soluble

Stability:

Solution: A solution of 100 mg/mL in water stored at room temperature for 44 days showed no decomposition.

Ultraviolet Absorption:

(0.1 N HC1)

max = 283 ± 2 nm           E = 36,200 - 40,000

High Performance Liquid Chromatography:

Column: 4 mm i.d. x 300 mm Spherisorb ODS

Mobile Phase: 4% acetonitrile in 0.1 M KH pH adjusted to 3.8 with H

Flow Rate: 2 mL/min

Detection: UV at 210 nm

Sample Preparation: 1 mg/mL in water or internal standard solution

Internal Standard: 1- naphthol -4- sulfonic acid Na salt (7 mg in 25 mL water)

Retention Volumes: 8.0 mL (NSC - 32946)
                  11.0 mL (I.S.)