LEUCOVORIN CALCIUM

NSC - 3590

SMILE String: n1c(c2N([CH](CNc2nc1N)CNc1ccc(cc1)C(=O)N[CH](CCC(O)=O)C(O)=O)C=O)O.[Ca+2]

CAS Registry Number: 1492-18-8

Molecular Formula: C20H23N7O7.Ca          M.W.: 511.5

Chemical Name: N Amino-5-formyl-1,4,5,6,7,8-hexahydro-4-oxo-6-pteridinyl)methyl]amino]benzoyl]-L-glutamic acid, calcium salt(1:1), (R,S)-

Other Names:
Citrovorum Factor; Folinic Acid; Calcium Leucovorin

Approximate Solubility:           (mg/mL)

DMSO                               « 1
H2O                                100
0.1 N NaOH                         < 20

Stability:

Bulk:

Calcium leucovorin was stable in bulk form after 4 weeks storage at 60 ° C (HPLC).

Solution: Leucovorin in 0.1 N NaOH (pH 13) and in deionized water (pH6) was stable at room temperature under laboratory illumination for at least 24 hours (HPLC).

Ultraviolet Absorption:

(0.1 N NaOH)

max=282 ± 2 nm         E = 23,000 - 26,500

High Performance Liquid Chromatography:

Column: 30 cm x 4 mm i.d. Spherisorb ODS

Mobile Phase: 15% McOH in 0.1 M KH pH adjusted to 4.0

Flow Rate: 1.5 mL /min

Detection: UV at 254 nm

Sample Preparation: 1 mg/mL in water or internal standard solution

Internal Standard: Folic acid (1 mg/mL in 0.02 N NaOH)

Retention Volume: 12.0 mL (NSC -3590)
                  20.2 mL (I.S.)

Optical Rotation:

(c = 1, H2O)   [a]20 D = 13.0 ± 2º

Toxicity Data:

Mouse(iv): LD50 732 mg/kg

Drugs in Japan. Ethical Drugs. 6,910,(1982)