HMBA

NSC - 95580

SMILE String: C(=O)(C)NCCCCCCNC(=O)C

Chemical Name: N,N'-Hexamethylenebisacetamide

CAS Registry Number: 3073-59-4

Molecular Formula: C10H20N2O2      M.W.: 200.3

Approximate Solubility:           (mg/mL)

H2O                                > 50
pH 4 Acetate buffer                > 50
pH 9 Carbonate buffer              > 50
0.1 N NaOH                         > 50
0.1 N HCL                          > 50
10% Ethanol                        > 50
95% Ethanol                        > 50
Methanol                           > 50

Stability:

Bulk: A sample stored at 60°C in the dark for 30 days retained 99 2% of its potency (GC).

Solution: Samples were dissolved in water at a concentration of 20 mg/mL and stored in clear glass containers at room temperatures under normal laboratory light for 9 days. The sample retained 98 2% of its potency (GC).

Gas - Liquid Chromatography:

Column: Packed glass, 10% OV-17 on Gas Chrom Q, 100/120 Mesh, 1.83 m x 2 mm i.d.

Carrier Gas: Helium

Flow Rate: 30 mL/min

Temperatures: Oven 205°C
              Injector 250°C
              Detector 250°C

Detection: FID

Retention Times: 6.4 min (NSC- 95580)
                 10.3 min (I.S.)

Sample Preparation: 1 mg/mL in internal standard solution

Internal Standard: Pentacosane (0.6 mg/mL in isopropanol/hexane, 70/30)