Genomics Technologies and Services

The OSTP has evaluated a number of genomic platforms and made these available to CCR investigators through agreements with various companies.  We have also arranged for full-service gene and miRNA expression profiling through companies and NCI core facilities.  These resources include:

1) Microarray and miRNA Platforms

Nanostring Technologies (available through either the CCR DNA Sequencing Minicore in building 37 or the Laboratory of Molecular Technologyhttp://web.ncifcrf.gov/atp/default.asp?LabID=10&page=labs , ATP, NCI-Frederick.

In addition to the arrays and services available from the companies listed above, a number of microarray scanners and hybridization stations have been installed in several locations for general access by CCR investigators.  Locations and type of equipment are as follows:

Affymetrix Scanner and Hybridization Stations

Agilent Scanners

Molecular Devices Scanners

A general scanner for scanning NimbleGen and other spotted arrays

2) DNA Sequencing

•   Illumina GA2x and HiSeq2000 sequencing technology: NCI Sequencing Facility currently being staffed at the Advanced Technology Center by SAIC. 

•   454 and Ion Torrent sequencing technology: Available services through the LMT*

•   Sanger sequencing technology, ABI 3730: Available services through the LMT*

•   ABI 3130XL fluorescence-based sequencer (DNA Sequencing MiniCore)**

•   Biotage PyroMark MD: Available services through the LMT*

*Laboratory of Molecular Technology
The LMT DNA Sequencing Laboratory provides standard and high-throughput sequencing, BAC sequencing, bi-sulfite sequencing, individual sample and next-generation 454 sequencing, and re-sequencing/mutation detection.

For more information contact:
Daniel Soppet, Ph.D.
Director, Laboratory of Molecular Technolgoy
Phone: 301-228-4447
E-mail: soppetdr@mail.nih.gov

**DNA Sequencing MiniCore
The DNA Sequencing MiniCore Facility is designed to provide NCI investigators with rapid processing of their DNA sequence samples. The core accepts samples for DNA sequencing or GeneMapper analysis. The facility analyzes these samples using ABI 3130XL fluorescence-based sequencers. The MiniCore provides this service for small-to-intermediate volume users.

The core also has Nanostring Technologies nCounter instrumentation and offers digital gene expression services to the CCR community. In addition, the core offers both a Stratagene Real-Time PCR and an Agilent Microarray system; these systems are available to CCR investigators to reserve and use. There is no cost for the use of these instruments.

For more information contact:
Kathy Hartman
Head, DNA MiniCore Facility
Building 37, Room 2135
37 Convent Drive
Bethesda, MD 20892
Phone: 301-594-9630
E-mail: kathleen_hartman@nih.gov

The OSTP provides a subsidy for purchases of arrays and gene expression profiling services. Please go to the OSTP Subsidy Request page for more information and to place a subsidy request.