Extraction of High Quality RNA - A Video Tutorial
Studies requiring gene expression analysis by microarray methods require the extraction of high quality RNA. Fresh, frozen tissue is the optimal sample for these studies. This collection of PDFs and Flash video provides detailed protocols along with animated procedures to assist the investigator in tissue collection, RNA extraction and RNA quality assessment. While techniques are demonstrated using the rat liver, the basic principles may be applied to most tissues regardless of species.
Section 508 Information: The PDF files outlined below are an accessible version of content provided in the non-accessible Flash video included in the ZIP file below.
Download Protocol PDF files
- Supplies (103KB)
- Instrument Cleaning - Rnase Decontamination (115KB)
- Disruption and Homogenization of Tissue for the Extraction of RNA (99KB)
- RNA Isolation from Pico-scale samples (1 cell (10-25pg) - 10mg tissue) with the PicoPure (101KB)
- RNA Isolation Using The Qiagen RNEasy Midi Kit (18KB)