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Stem Cell Information
The National Institutes of Health resource for stem cell research
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HomeResearch TopicsStem Cell Research at NIHNIH Stem Cell UnitFeeder-Free Culture of hESCs
Pluripotent stem cell colonies may be cultured feeder-free on 1.25% Matrigel in mTeSR1 medium (Stem Cell Technologies) according to the manufacturer's instructions or as outlined below.
Page citation: Feeder-Free Culture of hESCs. In Stem Cell Information [World Wide Web site]. Bethesda, MD: National Institutes of Health, U.S. Department of Health and Human Services, 2011 [cited Sunday, February 17, 2013] Available at <http://stemcells.nih.gov/research/nihresearch/scunit/pages/feederfree.aspx>