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NCJ Number:
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NCJ 199069
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Title:
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Automated STR Analysis for DNA Databases
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Series:
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NIJ Research Report
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Author(s):
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Mark W. Perlin Ph.D.
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Corporate Author:
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Cybergenetics
United States
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Date Published:
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12/2002 |
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Page Count:
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20 |
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Sponsoring Agency:
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Grant Number:
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2000-IJ-CX-K005 |
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Sale Source:
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NCJRS Photocopy Services
Box 6000
Rockville, MD 20849-6000
United States
Cybergenetics
160 North Craig Street, Suite 210
Pittsburgh, PA 15213
United States |
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Document:
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PDF |
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Agency Summary:
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Agency Summary |
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Publisher:
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http://perlin@cybgen.com |
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Type:
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Studies/research reports |
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Language:
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English |
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Country:
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United States |
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Annotation:
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This document focuses on the validation of automated short tandem repeat (STR) analyses of DNA databases. |
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Abstract:
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STR technology has enabled the rapid generation of DNA data for use in human identification. These data must be carefully analyzed. There is now an acute shortage of skilled data reviewers. The original STR data are carefully reviewed by two or more people to minimize error. To alleviate the critical labor shortage, the TrueAllele expert system was developed. The computer program automates virtually every human review function and provides consistent quality assessment and allele designation. The TrueAllele process begins with automated input of the data. The second step is automated gel image or capillary signal analysis. Step three is the allelic analysis, where the computer begins by analyzing the allelic ladders, transforming the data into DNA length coordinates and then accurately quantitating every DNA event in the data. In the final output step, TrueAllele automatically exports the data in format suitable for automated database import. A concordance validation study was conducted of the TrueAllele expert system for DNA database applications. Over 48,000 genotypes were processed using diverse STR panels and automated DNA sequences. It was demonstrated that the automated analysis and quality assurance system was accurate and that it could reduce the human time, error, effort, and costs associated with conventional labor-intensive review of Combined DNA Index System (CODIS) data. The automated methods were objective and comprehensive, providing for more uniform application of DNA forensic technology. 4 figures, 4 tables, 9 references |
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Main Term(s):
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Testing and measurement ; DNA fingerprinting |
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Index Term(s):
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Crime laboratories ; Trace evidence ; Suspect identification ; Blood and body fluid analysis ; Hair and fiber analysis ; Forensic sciences ; NIJ grant-related documents |
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To cite this abstract, use the following link:
https://www.ncjrs.gov/App/Publications/abstract.aspx?ID=199069
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* A link to the full-text document is provided whenever possible. For documents
not available online, a link to the publisher's web site is provided.
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