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Center for Cancer Research National Cancer Institute
Office of Science and Technology Partnerships

Collaborative Protein Technology Resource

Overview:

With the recent retirement of Paul Goldsmith, The Antibody and Protein Purification Unit (APPU), a collaborative CORE facility within the Office of Science and Technology Partnerships, has been reorganized into a new core service, The Collaborative Protein Technology Resource.  The group is now situated within the Laboratory of Cell Biology.

Supported Technologies:

NanoPro Technology:  The CPTR has extensive experience in the characterization of proteins for post-translational modifications and can assist PI's with the Nanopro isoelectric focusing immunoassays which can profile in 6-24 ng of total cell lysate protein the percent phosphorylation status of a given protein using antibody specific to the unmodified protein.  This extremely sensitive technique requires validated antibodies and the CPTR will provide the validation services as well as the actual performance of the assays.  Some consumables will be required from the researcher.

Simon Simple Western Assays, Capillary based automated Western system

-  Fully automated Westen system, highly quantitative data with excellent reproducibility

Contact information:

Jinqui (Jessie) Chen:
chenj13@mail.nih.gov
Telephone: 301-496-7917

Please click on the link below to download a summary of the CPTR capabilities.

PI retreat handout.ppt

Mass Spectrometry: 

-  Quantitative proteomics analyses
-  Identification of sites of post-translational modification
-  Identification of proteins in complexes, organelles, cellular fractions

Developing Technologies

-  Click chemistry-based affinity enrichment of proteins for analysis of post-translational modifications  and related protein interactors
-  Identification of phosphatase substrates

Contact:

Mass Spectrometry Program Manager:

Lisa Jenkins, PhD
Tel 301-496-4549
jenkinsl@mail.nih.gov

Added by Goldstein, David (NIH/NCI) [E] , last edited by Goldstein, David (NIH/NCI) [E] on Mar 24, 2012 12:45