Abstract
To assess markers of lung inflammation, we used SELDI-TOF and 2-DE to study changes in
bronchoalveolar lavage (BAL) protein in 33 subjects challenged with local bronchial lung endotoxin
and saline and in 11 patients with acute respiratory distress syndrome (ARDS). Differences
in the SELDI-TOF spectra were assessed by t-test after baseline subtraction, normalization to
total ion current and alignment by m/z calibration. The temporal changes in acute inflammatory
BAL (6, 24 and 48 h following endotoxin challenge) on hydrophobic binding chip surfaces
revealed the differential presence of proteins of 9, 14, 18 and 28 kDa (all p ,0.001) in the
inflammatory BAL. This differential pattern was also found in the ARDS BAL. Principal component
analysis of the entire SELDI-TOF spectra separated normal BAL, experimental and clinical
lung inflammation supporting the notion of a distinctive protein pattern associated with
acute lung inflammation. An analysis of the hydrophobic fraction of the inflammatory BAL
using 2-DE, identified increased levels of apolipoprotein A1, and S100 calcium-binding proteins
A8 and A9 in the inflammatory BAL. This pattern was also found in ARDS BAL after
immunoblot analysis. These approaches will be useful to improve current methods of monitoring
lung inflammation and to identify new therapeutic targets.
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