NCI Resources and Services

Name	Antibody Characterization Laboratory (Advanced Technology Program, SAIC-Frederick)
Expertise	Development and/or detailed quality control of monoclonal antibody reagents
Location	Building 433, Room 15, NCI-Frederick
Fee Structure

Name	Collaborative Protein Technology Resource, CCR
Expertise	Mass Spectrometry - quantitative proteomics analyses, identification of sites of post-translational modification, identification of proteins in complexes, organelles, and cellular fractions NanoPro, capillary-based capillary isoelectric focusing immunoassays - novel platform for studying cell signaling pathways and phospho-proteomics; simultaneously separate, detect and quantify multiple protein phosphorylation isoforms in limited cell populations, such as stem cells, primary cells, fine needle aspirates, other patient specimens, etc. Simon Simple Western Assays, capillary-based automated Western system - fully automated Westen system, highly quantitative data with excellent reproducibility
Location	Building 37, Room 2140, Bethesda
Fee Structure	Note: *Some costs are shared with collaborator.*

Name	Laboratory of Proteomics and Analytical Technologies (Advanced Technology Program, SAIC-Frederick)
Expertise	Mass spectrometry NMR spectroscopy In vivo protein-protein interactions Chromatography ICAT analysis In-gel digestion Phosphopeptide identification
Location	Building 469, Room 160, NCI-Frederick
Fee Structure	Note: *Collaborations are available based on the lab director's review and approval*.

Name	Protein Chemistry Laboratory (Advanced Technology Program, SAIC-Frederick)
Expertise	Molecular interaction analysis using surface plasmon resonance (BiaCore) Fluorescence spectroscopy HPLC purification/analysis N-terminal protein sequencing Mass spectroscopy Mass spectroscopy imaging
Location	Building 469, Room 241, NCI-Frederick
Fee Structure	Note: *Collaborations are available based on the lab director's review and approval*.

Name	Protein Expression Laboratory (Advanced Technology Program, SAIC-Frederick)
Expertise	Optimization of genes for protein expression and purification Small-scale parallel expression and purification testing Insect, mammalian, E. coli, yeast, cell-free expression Protein purification, micrograms to grams, low endotoxin Adenovirus and lentivirus construction, production, titer ELISA and qPCR assays for viruses in human samples Cell line testing for mycoplasma, pathogenic viruses, identity
Location	Building 327, Room 7, NCI-Frederick
Fee Structure	Note: *Collaborations are available based on the lab director's review and approval.*

Name	Protein Purification Core (Macromolecular Crystallography Laboratory, CCR)
Expertise	Protein purification and characterization Chemical modification of proteins Protein production
Location	Building 538, Room 248, NCI-Frederick
Fee Structure	Note: *This facility is dedicated to MCL principal investigators and their collaborators*.