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How to extract DNA from strawberries

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Uploaded on Oct 21, 2010

Drs. Eric Green and Carla Easter from the National Human Genome Research Institute (NHGRI) of the National Institutes of Health (NIH) demonstrate how to extract DNA from strawberries using everyday household items.

More information at: http://www.genome.gov/activities/

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Uploader Comments (GenomeTV)

  • ra1000elisa

    ra1000elisa 8 months ago

    How much DNA do you actually get?

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  • GenomeTV

    GenomeTV 8 months ago

    The amount of DNA that can be isolated depends on a number of thing: How well did you “smash” the strawberries? Did you make sure to add enough detergent and/or salt? How big was the strawberry?  How many strawberries did you use? Did you add enough or too much alcohol?

    All these things could affect the yield or amount of DNA you isolate.

    Carla

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    in reply to ra1000elisa (Show the comment)
  • Mary C

    Mary C 8 months ago

    How can I prove my teacher that I've got actual DNA? Is there anyway to prove it without a fancy microscope?

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  • GenomeTV

    GenomeTV 8 months ago

    One way to prove that what you have is DNA without a fancy microscope would be to use gel electrophoresis to analyze what you isolated. For more information on gel electrophoresis you can visit: learn.genetics.utah.edu/conten­t/labs/gel/. First, you would need to clean up the DNA you extracted because what is isolated from the strawberry is quite dirty; and one will get much better results from clean DNA.

    Carla

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    in reply to Mary C (Show the comment)
  • Mary C

    Mary C 8 months ago

    So basically I have to build my own electrophoresis chamber. So there's absolutely no other way I can prove that's DNA without it? I don't really have the money to buy UV light, which is required in the building of the chamber. I'm screwed, am I?

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    in reply to GenomeTV (Show the comment)
  • GenomeTV

    GenomeTV 8 months ago

    I totally understand your situation. Here is a link that highlight’s that others have had the same question:

    newton.dep.anl.gov/askasci/mol­e00/mole00687.htm

    Carla

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    in reply to Mary C (Show the comment)

All Comments (75)

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  • scarycatdog

    scarycatdog 2 days ago

    i watched this in class today

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  • Jo Lex

    Jo Lex 2 weeks ago

    Does it really have to be a strawberry?

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  • hrbeeli

    hrbeeli 3 weeks ago

    If you analized that "snot" you'd find that it is mostly denaturated protein. This and similar experiments in school give a wrong picture.

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  • TheLazyKovu

    TheLazyKovu 1 month ago

    Is it possible to use Meat Tenderizer, which has enzymes that break down proteins to get strands of DNA? The meat tenderizer that breaks down proteins is important because DNA in the nucleus is "tangled" up by histones. Histones are indeed made of proteins, and by breaking that down, you have pure DNA that are in strands.

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  • Jihad vv

    Jihad vv 1 month ago

    superb

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  • Raja Rb

    Raja Rb 1 month ago

    thats gross and informative, thanks :D

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  • CyberSword10

    CyberSword10 1 month ago

    I reccomend using pure Ethyl Alcohol (Ethanol).

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  • BeautyDark1996

    BeautyDark1996 3 months ago

    Is there any other thing that I can use except Alcohol ..?!

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  • NobodyXChallengerYT

    NobodyXChallengerYT 3 months ago

    Okay...this worked at first but the next day I couldn't get anything to precipitate. I did the exact same things as before.

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  • artsea1channel

    artsea1channel 4 months ago

    I found that using more salt is better

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