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| Microbiology Laboratory Guidebook |
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The Guidebook contains current protocols for analytical tests required by FSIS regulatory activities
on meat, poultry and egg products.1 Specifically, microbiological methods
are presented for sample preparation, isolation and identification
of the major foodborne pathogenic microorganisms and their toxins,
meat tissue species identification, and the detection of extraneous
materials and antimicrobial residues. Media and reagent formulations,
and Most Probable Number Tables are contained in an appendix. |
Receive email notification when the Microbiology Laboratory Guidebook is updated.
|
Methods Available on this Site:
| Method Number |
Method Title |
| |
Cover, Foreword, General Considerations (PDF Only) |
| 1. |
Sample Preparation For Meat, Poultry and
Pasteurized Egg Products (1998; PDF Only) |
| 2. |
Physical Examination of Meat and
Poultry Products (1998; PDF Only) |
| 3.01 |
Quantitative Analysis of Bacteria in Foods as
Sanitary Indicators (Jan 20, 2011; PDF Only) |
| 4.05 |
Isolation and Identification of Salmonella
from Meat, Poultry, Pasteurized Egg and Catfish Products (Jan 20, 2011; PDF Only)
|
| 4C.03 |
FSIS Procedure for the Use of a Polymerase
Chain Reaction (PCR) Assay for Screening Salmonella in Raw Meat Products, Raw Catfish Products, Carcass Sponge
Samples, Whole Bird Rinses, Ready-to-Eat Meat, Poultry Products, and Pasteurized Egg Products (Jan 20, 2011; PDF Only) |
| FLOW CHART |
Salmonella
spp.—Ready to Eat (RTE), Egg, Raw Ground Products, Catfish, Sponges, Rinses (Nov 15, 2010; PDF Only) |
| 5.06 |
Detection, Isolation and Identification of
Escherichia coli O157:H7 from Meat Products (Jun 4, 2012; PDF Only)
|
| 5A.02 |
FSIS Procedure for the Use of
Escherichia coli O157:H7 Screening Tests (Oct 1, 2010; PDF Only) |
| 5B.02 |
Detection and Isolation of non-O157
Shiga-toxin Producing Escherichia coli (STEC) from Meat Products (Jun 4, 2012; PDF Only)
- Appendix 1.01, Primer and Probe Sequences and Reagent Concentrations for non-O157 Shiga Toxin-Producing Escherichia coli (STEC) Real-Time PCR Assay (Jun 4, 2012; PDF Only)
- Appendix 2.01, Morphologies of Representative Strains from Six non-O157 Shiga Toxin-Producing Escherichia coli (STEC) Grown on Modified Rainbow Agar (Jun 4, 2012; PDF Only)
- Appendix 3.00, PCR Platform Instructions, Data Analysis, and Control Results Interpretation for non-O157 Shiga Toxin-Producing Escherichia coli (STEC) Real-time PCR Assay (Jun 4, 2012; PDF Only)
- Appendix 4.00, Non-O157 STEC Method Flow Chart (Jun 4, 2012; PDF Only)
|
| 7. |
Isolation and Identification of
Aeromonas Species from Meat and Poultry Products (1998; PDF Only) |
| 8.07 |
Isolation
and Identification of Listeria monocytogenes from Red
Meat, Poultry, Egg, and Environmental Samples (Aug 3, 2009; PDF Only) |
| 8A.04 |
FSIS
Procedure for the Use of a Listeria monocytogenes Polymerase Chain Reaction (PCR)
Screening Test (Aug 3, 2009; PDF Only) |
| FLOW CHART |
Listeria monocytogenes
in Meat and Poultry Ready-To-Eat Products, Egg Products, and Environmental Samples (Nov 15, 2010; PDF Only) |
| 9. |
Isolation
& Identification of Pathogenic Yersinia enterocolitica from Meat and Poultry Products (1998; PDF Only) |
| 10. |
Examination
of Heat Processed, Hermetically Sealed (Canned) Meat and Poultry Products (1998; PDF Only) |
| 11. |
Tests For Enzymes In Meat and Meat
Products (1998; PDF Only) |
| 12. |
Examination
of Meat and Poultry Products For Bacillus cereus (1998; PDF Only) |
| 13. |
Examination
of Meat and Poultry Products For Clostridium perfringens (1998; PDF Only) |
| 14. |
Methods
for the Detection of Clostridium botulinum Toxins In Meat and Poultry Products (1998; PDF Only) |
| 16. |
Agarose
Thin-Layer Isoelectric Focusing (TLIEF) For Species Determination of Raw Muscle Tissues (1998; PDF Only) |
| 17.02 |
Identification
of Animal Species in Meat and Poultry Products (Feb 10, 2005; PDF Only) |
| 18. |
Species
Identification Field Tests (SIFT) (1998; PDF Only) |
| 19. |
Competitive
Enzyme-Linked Immunoassay (CELIA) For The Detection and Quantitation of Chloramphenicol (1998; PDF Only) |
| |
Chapter Numbers 22-31 Reserved For Future Use. |
| 33. |
Detection
of Antimicrobial Residues In Meat and Poultry Tissue By Screen Tests (1998; PDF Only)
| Part A |
Detection of Antimicrobial
Residues By Swab Test On Premises (STOP) |
| Part B |
Detection of Antimicrobial Residue
In Calves By Calf Antibiotic and Sulfonamide Test (CAST) |
| Part C |
Tentative Confirmation of CAST
Results For Sulfonamide Residues In Meat and Poultry Tissue |
| Part D |
Detection of Antimicrobial Residue
By Fast Antimicrobial Screen Test (FAST) |
| Part E |
Evaluation of Antimicrobial Residues
In Meat and Poultry Tissue By A Modified Fast Antimicrobial
Screen Test (M-FAST) |
|
| 34.03 |
Bioassay
for the Detection, Identification and Quantitation of Antimicrobial
Residues in Meat and Poultry Tissue (May 25, 2011; PDF Only) |
| 35. |
Detection
of Antibiotic and Sulfonamide Residues In Meat Tissue By Commercially Available Immunoassay Kits (1998; PDF Only) |
| 40.00 |
Avian Influenza Detection
in the Chicken Heart Using Real-Time Reverse Transcriptase PCR (Oct 8, 2007; PDF Only) |
| 41.01 |
Isolation,
Identification, and Enumeration of Campylobacter jejuni/coli/lari
from Poultry Rinse and Sponge Samples (Aug 1, 2011; PDF Only) |
| FLOW CHARTS |
Isolation, Identification, and Enumeration of Campylobacter jejuni/coli/lari
from Poultry Rinse and Sponge Samples
|
| TABLE |
FSIS
Laboratory Regulatory Sample Pathogen Methods Table and Definitions (Jan 31, 2012; PDF Only) |
| Appendices: |
| 1.06 |
Media and Reagents (Nov 4, 2011; PDF Only) |
| 2.03 |
Most Probable Number Procedure and
Tables (Jan 28, 2008; PDF Only) |
|
| 1 The methods described
in this guidebook are for use by the FSIS laboratories. FSIS does
not specifically endorse any of the mentioned test products and acknowledges
that equivalent products may be available for laboratory use. |
Last Modified: June 7, 2012 |
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